Abstract：
DNA nanotubes with controllable geometries hold a wide range of interdisciplinary applications. When preparing DNA nanotubes of varying widths or distinct chirality, existing methods require repeatedly designing and synthesizing specific DNA sequences, which can be costly and laborious. Here, we proposed an intercalator-assisted DNA tile assembly method which enables the production of DNA nanotubes of diverse widths and chirality using identical DNA strands. Through adjusting the concentration of intercalators during assembly, the twisting direction and extent of DNA tiles could be modulated, leading to the formation of DNA nanotubes featuring controllable widths and chirality. Moreover, through introducing additional intercalators and secondary annealing, right-handed nanotubes could be reconfigured into distinct left-handed nanotubes. We expect that this method could be universally applied to modulating the self-assembly pathways of various DNA tiles and other chiral materials, advancing the landscape of DNA tile assembly.
摘要：
具有可控几何形状的DNA纳米管具有广泛的跨学科应用。当制备不同宽度或不同手性的DNA纳米管时，现有的方法需要反复设计和合成特定的DNA序列，这可能是昂贵和费力的。这里,我们提出了一种嵌入剂辅助的DNA瓦片组装方法，该方法可以使用相同的DNA链生产不同宽度和手性的DNA纳米管。通过在组装过程中调节嵌入剂的浓度，可以调节DNA瓦片的扭曲方向和程度，导致形成具有可控宽度和手性的DNA纳米管。此外，通过引入额外的嵌入剂和二次退火，右旋纳米管可以重新配置成不同的左旋纳米管。我们期望这种方法可以普遍应用于调节各种DNA瓦片和其他手性材料的自组装途径，推进DNA瓷砖组装的景观。
