Abstract：
Despite the diversity of microbiota in birds is similar to that of other animals, there is a lack of research on the gut microbial diversity of nondomesticated bird species. This study aims to address this gap in knowledge by analyzing the bacterial communities present in the gut of two important game bird species, the Ring-necked pheasant (Phasianus colchicus) and the Green pheasant (Phasianus versicolor) to understand the gut microbial diversity of these species. The gut microbiome of 10 individual pheasants from two different species was studied using pooled fecal samples. We used 16S rRNA gene sequencing on the Ion S5 XL System next-generation sequencing with Mothur and SILVA Database for taxonomic division. An average of 141 different operational taxonomic units were detected in the gut microbiome. Analysis of microbial classification revealed the presence of 191 genera belonging to 12 different phyla in both pheasants. Alpha diversity indices revealed that P. colchicus exhibited most prevalence firmicutes with bacillus species microbial community than P. versicolor. Alpha diversity indices indicated that P. colchicus had a more diverse community and P. versicolor had a greater diversity of evolutionary lineages, while both species had similar levels of species richness and sample inclusiveness. These findings may have implications for the health and well-being of pheasants, serving as a reference for their bacterial diversity. Additionally, they provide a baseline for future research and conservation efforts aimed at improving the health and well-being of these and possibly other avian species.
摘要：
尽管鸟类的微生物群多样性与其他动物相似，缺乏对非驯化鸟类肠道微生物多样性的研究。这项研究旨在通过分析两种重要的猎鸟物种肠道中存在的细菌群落来解决这一知识差距，环颈野鸡（Phasianuscolchicus）和绿色野鸡（Phasianusversicolor）了解这些物种的肠道微生物多样性。使用合并的粪便样品研究了来自两个不同物种的10只野鸡的肠道微生物组。我们在带有Mothur和SILVA数据库的IonS5XL系统下一代测序上使用16SrRNA基因测序进行分类。在肠道微生物组中平均检测到141个不同的操作分类单位。对微生物分类的分析表明，在两种野鸡中都存在191个属，属于12个不同的门。Alpha多样性指数显示，与杂色疟原虫相比，秋水疟原虫在芽孢杆菌微生物群落中的流行率最高。Alpha多样性指数表明，colchicus具有更多样化的群落，而versicolor具有更大的进化谱系多样性。而两个物种的物种丰富度和样本包容性水平相似。这些发现可能对野鸡的健康和福祉有影响，作为其细菌多样性的参考。此外,它们为未来的研究和保护工作提供了基线，旨在改善这些和可能的其他鸟类的健康和福祉。
