PDF(Pubmed)
Abstract：
Bioactive polysaccharides and oligosaccharides were successfully extracted from three distinct seaweeds: Sargassum sp., Graciallaria sp., and Ulva sp. utilizing various extraction techniques. The obtained polysaccharides and oligosaccharides were subjected to comprehensive characterization, and their potential antioxidant properties were assessed using a Hep G2 cell model. Analysis via FTIR spectroscopy unveiled the presence of sulfate groups in the polysaccharides and oligosaccharides derived from Sargassum sp. The antioxidant capabilities were assessed through various assays (DPPH, ABTS, Fe-ion chelation, and reducing power), revealing that SAR-OSC exhibited superior antioxidant activity than others. This was attributed to its higher phenolic content (24.6 μg/mg), FRAP value (36 μM Vitamin C/g of extract), and relatively low molecular weight (5.17 kDa). The study also investigated the protective effects of these polysaccharides and oligosaccharides against oxidative stress-induced damage in Hep G2 cells by measuring ROS production and intracellular antioxidant enzyme expressions (SOD, GPx, and CAT). Remarkably, SAR-OSC demonstrated the highest efficacy in protecting Hep G2 cells reducing ROS production and downregulating SOD, GPx, and CAT expressions. Current findings have confirmed that the oligosaccharides extracted by the chemical method show higher antioxidant activity, particularly SAR-OSC, and robust protective abilities in the Hep G2 cells.
摘要：
从三种不同的海藻中成功提取了生物活性多糖和寡糖：Sargassumsp。,Graciallariasp.,和Ulvasp.利用各种提取技术。对所得多糖和寡糖进行了全面表征,使用HepG2细胞模型评估其潜在的抗氧化性能。通过FTIR光谱分析揭示了源自Sargassumsp的多糖和寡糖中存在硫酸基团。通过各种测定(DPPH，ABTS,Fe-离子螯合,并降低功率)，表明SAR-OSC表现出比其他更好的抗氧化活性。这归因于其较高的酚类含量（24.6μg/mg），FRAP值（36μM维生素C/g提取物），和相对低的分子量(5.17kDa)。该研究还通过测量ROS的产生和细胞内抗氧化酶的表达（SOD，GPx,和CAT）。值得注意的是,SAR-OSC在保护HepG2细胞减少ROS产生和下调SOD方面表现出最高功效，GPx,和CAT表达式。目前的研究结果已经证实，通过化学方法提取的寡糖显示出更高的抗氧化活性，特别是SAR-OSC，在HepG2细胞中具有强大的保护能力。
