PDF(Pubmed)
Abstract：
Axial spondyloarthritis (axSpA) is characterized by type-17 immune-driven joint inflammation, and intestinal inflammation is present in around 70% of patients. In this study, we asked whether axSpA stool contained Th17-associated cytokines and whether this related to systemic Th17 activation. We measured stool cytokine and calprotectin levels by ELISA and found that patients with axSpA have increased stool IL-17A, IL-23, GM-CSF, and calprotectin. We further identified increased levels of circulating IL-17A+ and IL-17F+ T-helper cell lymphocytes in patients with axSpA compared to healthy donors. We finally assessed stool metabolites by unbiased nuclear magnetic resonance spectroscopy and found that multiple stool amino acids were negatively correlated with stool IL-23 concentrations. These data provide evidence of type-17 immunity in the intestinal lumen, and suggest its association with microbial metabolism in the intestine.
摘要：
轴向脊柱关节炎（axSpA）的特征是17型免疫驱动的关节炎症，约70%的患者存在肠道炎症。在这项研究中,我们询问axSpA粪便中是否含有Th17相关细胞因子,以及这是否与全身Th17激活有关.我们通过ELISA检测粪便细胞因子和钙卫蛋白水平，发现axSpA患者粪便IL-17A升高，IL-23,GM-CSF,还有钙卫蛋白.我们进一步确定了与健康供体相比，axSpA患者中循环IL-17A和IL-17FT辅助细胞淋巴细胞的水平升高。我们最终通过无偏倚的核磁共振波谱评估了粪便代谢物，发现多种粪便氨基酸与粪便IL-23浓度呈负相关。这些数据提供了肠腔17型免疫的证据，并提示其与肠道微生物代谢有关。
