PDF(Pubmed)
Abstract：
Introduction: Aged-related brain damage and gut microbiome disruption are common. Research affirms that modulating the microbiota-gut-brain axis can help reduce age-related brain damage. Methods: Ginseng, esteemed in traditional Chinese medicine, is recognized for its anti-aging capabilities. However, previous Ginseng anti-aging studies have largely focused on diseased animal models. To this end, efforts were hereby made to explore the potential neuroprotective effects of fecal microbiota transplantation (FMT) from Ginseng-supplemented aged mice to those pre-treated with antibiotics. Results: As a result, FMT with specific modifications in natural aging mice improved animal weight gain, extended the telomere length, anti-oxidative stress in brain tissue, regulated the serum levels of cytokine, and balanced the proportion of Treg cells. Besides, FMT increased the abundance of beneficial bacteria of Lachnospiraceae, Dubosiella, Bacteroides, etc. and decreased the levels of potential pathogenic bacteria of Helicobacter and Lachnoclostridium in the fecal samples of natural aged mice. This revealed that FMT remarkably reshaped gut microbiome. Additionally, FMT-treated aged mice showed increased levels of metabolites of Ursolic acid, β-carotene, S-Adenosylmethionine, Spermidine, Guanosine, Celecoxib, Linoleic acid, etc., which were significantly positively correlated with critical beneficial bacteria above. Additionally, these identified critical microbiota and metabolites were mainly enriched in the pathways of Amino acid metabolism, Lipid metabolism, Nucleotide metabolism, etc. Furthermore, FMT downregulated p53/p21/Rb signaling and upregulated p16/p14, ATM/synapsin I/synaptophysin/PSD95, CREB/ERK/AKT signaling in brain damage following natural aging. Discussion: Overall, the study demonstrates that reprogramming of gut microbiota by FMT impedes brain damage in the natural aging process, possibly through the regulation of microbiota-gut-brain axis.
摘要：
简介：与老年人相关的脑损伤和肠道微生物组破坏很常见。研究证实，调节微生物群-肠-脑轴可以帮助减少与年龄相关的脑损伤。方法:人参,受人尊敬的中医，以其抗衰老能力而闻名。然而,以前的人参抗衰老研究主要集中在患病的动物模型上。为此,因此，我们努力探索补充人参的老年小鼠粪便微生物群移植（FMT）对抗生素预处理的小鼠的潜在神经保护作用。结果：结果，在自然衰老小鼠中进行特定修饰的FMT改善了动物体重增加，延长端粒长度，脑组织抗氧化应激，调节细胞因子的血清水平，平衡Treg细胞的比例.此外,FMT增加了虎尾草科有益菌的丰度，Dubosiella,拟杆菌,等。并降低了自然衰老小鼠粪便样本中潜在致病菌螺杆菌和幼虫的水平。这表明FMT显著地重塑了肠道微生物组。此外,FMT处理的老年小鼠显示熊果酸代谢物水平升高，β-胡萝卜素,S-腺苷甲硫氨酸，亚精胺,鸟苷,塞来昔布,亚油酸,等。,与上述关键有益菌呈显著正相关。此外,这些确定的关键微生物群和代谢产物主要富集在氨基酸代谢途径中，脂质代谢，核苷酸代谢，等。此外，FMT下调p53/p21/Rb信号并上调p16/p14、ATM/突触素I/突触素/PSD95、CREB/ERK/AKT信号在自然衰老后脑损伤中的作用。讨论：总的来说，这项研究表明，FMT对肠道微生物群的重编程阻碍了自然衰老过程中的脑损伤，可能是通过调节微生物群-肠-脑轴。
