PDF(Pubmed)
Abstract：
While poly (3-hydroxybutyrate) (PHB) holds promise as a bioplastic, its commercial utilization has been hampered by the high cost of raw materials. However, glycerol emerges as a viable feedstock for PHB production, offering a sustainable production approach and substantial cost reduction potential. Glycerol stands out as a promising feedstock for PHB production, offering a pathway toward sustainable manufacturing and considerable cost savings. The identification and characterization of strains capable of converting glycerol into PHB represent a pivotal strategy in advancing PHB production research. In this study, we isolated a strain, Ralstonia sp. RRA (RRA). The strain exhibits remarkable proficiency in synthesizing PHB from glycerol. With glycerol as the carbon source, RRA achieved a specific growth rate of 0.19 h-1, attaining a PHB content of approximately 50% within 30 h. Through third-generation genome and transcriptome sequencing, we elucidated the genome composition and identified a total of eight genes (glpR, glpD, glpS, glpT, glpP, glpQ, glpV, and glpK) involved in the glycerol metabolism pathway. Leveraging these findings, the strain RRA demonstrates significant promise in producing PHB from low-cost renewable carbon sources.
摘要：
虽然聚（3-羟基丁酸酯）（PHB）有望成为生物塑料，由于原材料的高成本，其商业利用受到了阻碍。然而,甘油成为PHB生产的可行原料，提供可持续的生产方法和大幅降低成本的潜力。甘油是PHB生产的有前途的原料，为可持续制造和可观的成本节约提供了途径。能够将甘油转化为PHB的菌株的鉴定和表征代表了推进PHB生产研究的关键策略。在这项研究中,我们分离了一个菌株，Ralstoniasp.RRA（RRA）。该菌株在从甘油合成PHB方面表现出显著的熟练程度。以甘油为碳源，RRA实现了0.19h-1的特定生长速率，在30小时内达到了约50％的PHB含量。通过第三代基因组和转录组测序，我们阐明了基因组组成，并鉴定了总共八个基因(glpR，glpD,glpS,glpT,glpP,glpQ,glpV,和glpK）参与甘油代谢途径。利用这些发现，菌株RRA在从低成本可再生碳源生产PHB方面显示出显著的前景。
