PDF(Pubmed)
Abstract：
Intraspecific genetic variation is important for the assessment of organisms\' resistance to changing environments and anthropogenic pressures. Aquatic DNA metabarcoding provides a non-invasive method in biodiversity research, including investigations at the within-species level. Through the analysis of eDNA samples collected from the Peter the Great Gulf of the Japan Sea, in this study, we aimed to evaluate the identification of Amplicon Sequence Variants (ASVs) in marine eDNA among abundant species of the Zostera sp. community: Hexagrammos octogrammus, Pholidapus dybowskii (Teleostei: Perciformes), and Pandalus latirostris (Arthropoda: Decapoda). These species were collected from two distant locations to produce mock communities and gather aquatic eDNA both on the community and individual level. Our approach highlights the efficacy of eDNA metabarcoding in capturing haplotypic diversity and the potential for this methodology to track genetic diversity accurately, contributing to conservation efforts and ecosystem management. Additionally, our results elucidate the impact of nuclear mitochondrial DNA segments (NUMTs) on the reliability of metabarcoding data, indicating the necessity for cautious interpretation of such data in ecological studies. Moreover, we analyzed 83 publicly available COI sequence datasets from common groups of multicellular organisms (Mollusca, Echinodermata, Crustacea, Polychaeta, and Actinopterygii). The results reflect the decrease in population diversity that arises from using the metabarcode compared to the COI barcode.
摘要：
种内遗传变异对于评估生物体对不断变化的环境和人为压力的抵抗力很重要。水生DNA元编码为生物多样性研究提供了一种非侵入性方法，包括物种内的调查。通过对日本海彼得大湾的eDNA样本进行分析,在这项研究中，我们旨在评估Zosterasp.丰富物种中海洋eDNA中扩增子序列变体（ASV）的鉴定。社区：Hexagrammosoctogrammus，Pholidapusdybowskii（Teleostei：Perciformes），和Pandaluslatirostris（节肢动物：Decapoda）。这些物种是从两个遥远的地方收集的，以产生模拟群落，并在群落和个体水平上收集水生eDNA。我们的方法突出了eDNA元编码在捕获单倍型多样性方面的功效，以及这种方法准确跟踪遗传多样性的潜力，为保护工作和生态系统管理做出贡献。此外,我们的结果阐明了核线粒体DNA片段（NUMTs）对代谢编码数据可靠性的影响，表明在生态研究中对此类数据进行谨慎解释的必要性。此外，我们分析了来自多细胞生物常见群体的83个公开可用的COI序列数据集(Mollusca，棘皮病,甲壳动物,Polychaeta,和Actinopterygii）。结果反映了与COI条形码相比，使用元条形码引起的种群多样性减少。
