METHODS: Different identification panels and panel cells were created by RhD-positive red blood cells (RBCs) and RhD-negative RBCs, according to the underlying antibodies. Diluted anti-D reagent was used as simulated plasma for identification.
RESULTS: The antibody identification of single antibody with dose-effect and two antibodies present at the same time were successfully simulated.
CONCLUSIONS: It is a practical and cheap method for antibody identification training to use RhD blood group, especially when positive samples are short.
方法:由RhD阳性红细胞(RBC)和RhD阴性红细胞创建不同的鉴定面板和面板细胞,根据潜在的抗体。使用稀释的抗D试剂作为模拟血浆进行鉴定。
结果:成功模拟了具有剂量效应的单一抗体和同时存在的两种抗体的抗体鉴定。
结论:使用RhD血型进行抗体鉴定训练是一种实用而廉价的方法,特别是当阳性样本很短时。