Abstract：
In this study, we wanted to investigate the effectiveness of combining disease-modifying anti-rheumatic drugs (DMARD) with hyperbaric oxygen therapy (HBOT) in reducing inflammation in a rheumatoid arthritis (RA) model using rats. We divided 56 male Sprague-Dawley rats into seven groups and induced RA using complete Freund\'s adjuvant. Some groups received HBOT, whereas others were given etanercept or leflunomide. We started the treatment on the 10th day after inducing RA and continued it for 18 days. To evaluate the effectiveness of the treatments, we measured paw swelling and used X-rays to examine the joints before and after the treatment. We also analysed the levels of two inflammatory markers, tumour necrosis factor (TNF)-α and interleukin (IL)-1β, using an enzyme-linked immunosorbent assay. Additionally, we conducted histological analysis and assessed the expressions of anti-IL-1β and anti-TNF-α antibodies. All the treatment groups showed a significant decrease in arthritis scores, paw swelling and levels of TNF-α and IL-1β. The X-ray images revealed improvements in joint structure, and the histopathological analysis showed reduced inflammation and collagen abnormalities. Combining DMARD with HBOT had similar effects to individual therapies, suggesting a cost-effective and potentially safer approach for improving outcomes in rats with RA.
摘要：
在这项研究中,我们希望研究在类风湿关节炎(RA)大鼠模型中联合应用疾病缓解抗风湿药(DMARD)和高压氧治疗(HBOT)减轻炎症的效果.我们将56只雄性Sprague-Dawley大鼠分为7组，并使用完全弗氏佐剂诱导RA。一些团体接受了HBOT，而其他人则给予依那西普或来氟米特。我们在诱导RA后第10天开始治疗并持续18天。为了评估治疗的有效性，我们测量了爪肿胀，并在治疗前后使用X射线检查了关节。我们还分析了两种炎症标志物的水平，肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β，使用酶联免疫吸附测定。此外,我们进行了组织学分析，并评估了抗IL-1β和抗TNF-α抗体的表达。所有治疗组关节炎评分均有显著下降,爪肿胀和TNF-α和IL-1β水平。X射线图像显示关节结构有所改善，组织病理学分析显示炎症和胶原异常减少。将DMARD与HBOT结合使用与单独治疗具有相似的效果，提示改善RA大鼠预后的成本有效且可能更安全的方法。
