PDF(Pubmed)
Abstract：
Extracellular vesicles (EVs) derived from dental pulp stem cells (DPSC) have been shown an excellent efficacy in a variety of disease models. However, current production methods fail to meet the needs of clinical treatment. In this study, we present an innovative approach to substantially enhance the production of \'Artificial Cell-Derived Vesicles (ACDVs)\' by extracting and purifying the contents released by the DPSC lysate, namely intracellular vesicles. Comparative analysis was performed between ACDVs and those obtained through ultracentrifugation. The ACDVs extracted from the cell lysate meet the general standard of EVs and have similar protein secretion profile. The new ACDVs also significantly promoted wound healing, increased or decreased collagen regeneration, and reduced the production of inflammatory factors as the EVs. More importantly, the extraction efficiency is improved by 16 times compared with the EVs extracted using ultracentrifuge method. With its impressive attributes, this new subtype of ACDVs emerge as a prospective candidate for the future clinical applications in regenerative medicine.
摘要：
源自牙髓干细胞（DPSC）的细胞外囊泡（EV）已在多种疾病模型中显示出优异的功效。然而,目前的生产方法不能满足临床治疗的需要。在这项研究中,我们提出了一种创新的方法，通过提取和纯化由DPSC裂解物释放的内容物，大大提高了人工细胞来源的囊泡（ACDV）的生产，即细胞内囊泡。在ACDV和通过超速离心获得的ACDV之间进行比较分析。从细胞裂解物提取的ACDV符合EV的一般标准并且具有相似的蛋白质分泌谱。新型ACDV还显著促进伤口愈合，增加或减少胶原蛋白再生，并减少了作为电动汽车的炎症因子的产生。更重要的是,与超速离心法提取的电动汽车相比，提取效率提高了16倍。凭借其令人印象深刻的属性，这种新的ACDV亚型成为未来再生医学临床应用的潜在候选者.
