PDF(Pubmed)
Abstract：
Aedes aegypti and Aedes albopictus are the main vectors of arboviruses such as Dengue, Chikungunya and Zika, causing a major impact on global economic and public health. The main way to prevent these diseases is vector control, which is carried out through physical and biological methods, in addition to environmental management. Although chemical insecticides are the most effective strategy, they present some problems such as vector resistance and ecotoxicity. Recent research highlights the potential of the imidazolium salt \"1-methyl-3-octadecylimidazolium chloride\" (C18MImCl) as an innovative and environmentally friendly solution against Ae. aegypti. Despite its promising larvicidal activity, the mode of action of C18MImCl in mosquito cells and tissues remains unknown. This study aimed to investigate its impacts on Ae. aegypti larvae and three cell lines of Ae. aegypti and Ae. albopictus, comparing the cellular effects with those on human cells. Cell viability assays and histopathological analyses of treated larvae were conducted. Results revealed the imidazolium salt\'s high selectivity (> 254) for mosquito cells over human cells. After salt ingestion, the mechanism of larval death involves toxic effects on midgut cells. This research marks the first description of an imidazolium salt\'s action on mosquito cells and midgut tissues, showcasing its potential for the development of a selective and sustainable strategy for vector control.
摘要：
埃及伊蚊和白纹伊蚊是登革热等虫媒病毒的主要载体,基孔肯雅和寨卡,对全球经济和公共卫生造成重大影响。预防这些疾病的主要方法是病媒控制，这是通过物理和生物方法进行的，除了环境管理。虽然化学杀虫剂是最有效的策略，它们提出了一些问题，如媒介抗性和生态毒性。最近的研究强调了咪唑鎓盐“1-甲基-3-十八烷基咪唑鎓氯化物”（C18MImCl）作为针对Ae的创新和环保解决方案的潜力。埃及伊蚊.尽管它有很好的杀幼虫活动，C18MImCl在蚊子细胞和组织中的作用方式仍然未知。本研究旨在探讨其对Ae的影响。埃及伊蚊幼虫和Ae的三个细胞系。埃及伊蚊和Ae.白纹,将细胞效应与对人类细胞的影响进行比较。对处理的幼虫进行细胞活力测定和组织病理学分析。结果表明，咪唑盐对蚊子细胞的选择性比人类细胞高（>254）。食盐后，幼虫死亡的机制涉及对中肠细胞的毒性作用。这项研究首次描述了咪唑盐对蚊子细胞和中肠组织的作用，展示其在制定有选择和可持续的病媒控制战略方面的潜力。
