Abstract：
Controllable heparin-release is of great importance and necessity for the precise anticoagulant regulation. Efforts have been made on designing heparin-releasing systems, while, it remains a great challenge for gaining the external-stimuli responsive heparin-release in either intravenous or catheter delivery. In this study, an azobenzene-containing ammonium surfactant is designed and synthesized for the fabrication of photoresponsive heparin ionic complexes through the electrostatic complexation with heparin. Under the assistance of photoinduced trans-cis isomerization of azobenzene, the obtained heparin materials perform reversible athermal phase transition between ordered crystalline and isotropic liquid state at room temperature. Compared to the ordered state, the formation of isotropic state can effectively improve the dissolving of heparin from ionic materials in aqueous condition, which realizes the photo-modulation on the concentration of free heparin molecules. With good biocompatibility, such a heparin-releasing system addresses photoresponsive anticoagulation in both in vitro and in vivo biological studies, confirming its great potential clinical values. This work provides a new designing strategy for gaining anticoagulant regulation by light, also opening new opportunities for the development of photoresponsive drugs and biomedical materials based on biomolecules.
摘要：
可控的肝素释放对于精确的抗凝剂调节具有重要意义和必要性。努力设计肝素释放系统,while,在静脉或导管输送过程中获得外部刺激反应性肝素释放仍然是一个巨大的挑战.在这项研究中,设计并合成了一种含偶氮苯的铵表面活性剂，用于通过与肝素的静电络合制备光响应性肝素离子络合物。在偶氮苯的光诱导反式顺式异构化的帮助下，获得的肝素材料在室温下在有序晶体和各向同性液态之间进行可逆的无热相变。与有序状态相比，各向同性态的形成可以有效地提高肝素在水性条件下从离子物质中的溶解,实现了对游离肝素分子浓度的光调制。具有良好的生物相容性，这种肝素释放系统在体外和体内生物学研究中都解决了光响应性抗凝，证实了其巨大的潜在临床价值。这项工作提供了一种新的设计策略，通过光获得抗凝调节，也为基于生物分子的光敏药物和生物医学材料的开发开辟了新的机遇。
