Abstract：
Tris(2-chloroethyl) phosphate (TCEP), a chlorinated organophosphate ester, is commonly found in aquatic environments. Due to its various toxic effects, it may pose a risk to the health of aquatic organisms. However, the potential impacts of TCEP exposure on the intestinal microbiota and hepatic function in amphibians have not been reported. This study investigated the impact of long-term exposure to environmentally relevant concentrations of TCEP (0, 3, and 90 μg/L) on the intestinal microbiota and hepatic transcriptome of Polypedates megacephalus tadpoles. The results showed that the body size of the tadpoles decreased significantly with an increase in TCEP concentration. Additionally, TCEP exposure affected the diversity and composition of the intestinal microbiota in tadpoles, leading to significant changes in the relative abundance of certain bacterial groups (the genera Aeromonas decreased and Citrobacter increased) and potentially promoting a more even distribution of microbial species, as indicated by a significant increase in the Simpson index. Moreover, the impact of TCEP on hepatic gene expression profiles in tadpoles was significant, with the majority of differentially expressed genes (DEGs) (709 out of 906 total DEGs in 3 μg/L of TCEP versus control, and 344 out of 387 DEGs in 90 μg/L of TCEP versus control) being significantly down-regulated, which were primarily related to immune response and immune system process. Notably, exposure to TCEP significantly reduced the relative abundance of the genera Aeromonas and Cetobacterium in the tadpole intestine. This reduction was positively correlated with the down-regulated expression of immune-related genes in the liver of corresponding tadpoles. In summary, these findings provide empirical evidence of the potential health risks to tadpoles exposed to TCEP at environmentally relevant concentrations.
摘要：
磷酸三(2-氯乙基)酯(TCEP),一种氯化有机磷酸酯，常见于水生环境。由于其各种毒性作用，它可能对水生生物的健康构成威胁。然而,TCEP暴露对两栖动物肠道菌群和肝功能的潜在影响尚未有报道.这项研究调查了长期暴露于环境相关浓度的TCEP（0、3和90μg/L）对多足动物大头t的肠道微生物群和肝转录组的影响。结果表明，随着TCEP浓度的增加，t的体型显着减小。此外,TCEP暴露会影响t肠道菌群的多样性和组成，导致某些细菌群的相对丰度发生显着变化（气单胞菌属减少，柠檬酸杆菌增加），并可能促进微生物物种的更均匀分布，辛普森指数显著上升。此外，TCEP对t肝脏基因表达谱的影响是显著的，与大多数差异表达基因（DEGs）（在3μg/L的TCEP中906个总DEGs中有709个与对照相比，与对照相比，90μg/L的TCEP中的387个DEG中有344个被显著下调），主要与免疫反应和免疫系统过程有关。值得注意的是,暴露于TCEP可显着降低t肠中气单胞菌属和细菌属的相对丰度。这种减少与相应t的肝脏中免疫相关基因的表达下调呈正相关。总的来说，这些发现提供了实验证据,证明在环境相关浓度下暴露于TCEP对t的潜在健康风险.
