Abstract：
Basolateral amygdala (BLA) is a key hub for affect in the brain,1,2,3 and dysfunction within this area contributes to a host of psychiatric disorders.4,5 BLA is extensively and reciprocally interconnected with frontal cortex,6,7,8,9 and some aspects of its function are evolutionarily conserved across rodents, anthropoid primates, and humans.10 Neuron density in BLA is substantially lower in primates compared to murine rodents,11 and frontal cortex (FC) is dramatically expanded in primates, particularly the more anterior granular and dysgranular areas.12,13,14 Yet, how these anatomical differences influence the projection patterns of single BLA neurons to frontal cortex across rodents and primates is unknown. Using a barcoded connectomic approach, we assessed the single BLA neuron connections to frontal cortex in mice and macaques. We found that BLA neurons are more likely to project to multiple distinct parts of FC in mice than in macaques. Further, while single BLA neuron projections to nucleus accumbens were similarly organized in mice and macaques, BLA-FC connections differed substantially. Notably, BLA connections to subcallosal anterior cingulate cortex (scACC) in macaques were least likely to branch to other medial frontal cortex areas compared to perigenual ACC (pgACC). This pattern of connections was reversed in the mouse homologues of these areas, infralimbic and prelimbic cortex (IL and PL), mirroring functional differences between rodents and non-human primates. Taken together, these results indicate that BLA connections to FC are not linearly scaled from mice to macaques and instead the organization of single-neuron BLA connections is distinct between these species.
摘要：
基底外侧杏仁核（BLA）是影响大脑的关键枢纽，1,2,3和该区域内的功能障碍会导致许多精神疾病。4,5BLA与额叶皮层广泛且相互联系，6,7,8,9及其功能的某些方面在啮齿动物中在进化上是保守的，类人猿灵长类动物，和人类。10与鼠啮齿动物相比，灵长类动物中BLA的神经元密度大大降低，11和额叶皮层（FC）在灵长类动物中急剧扩大，特别是更前的颗粒和颗粒异常区域。12,13,14然而，这些解剖学差异如何影响单个BLA神经元在啮齿动物和灵长类动物之间向额叶皮质的投影模式尚不清楚.使用条形码连接体方法，我们评估了小鼠和猕猴中单个BLA神经元与额叶皮质的连接。我们发现，与猕猴相比，小鼠的BLA神经元更有可能投射到FC的多个不同部分。Further,而单个BLA神经元投射到伏隔核的组织在小鼠和猕猴中相似，BLA-FC连接有很大差异。值得注意的是,与周生ACC（pgACC）相比，BLA与猕猴的call下前扣带回皮层（scACC）的连接最不可能分支到其他内侧额叶皮层区域。在这些区域的小鼠同源物中，这种连接模式是相反的，下边缘和前边缘皮质（IL和PL），反映了啮齿动物和非人灵长类动物之间的功能差异。一起来看,这些结果表明，BLA与FC的连接不是从小鼠到猕猴的线性扩展，而是在这些物种之间，单神经元BLA连接的组织是不同的。
