Abstract：
Citri Reticulatae Pericarpium (CRP) is used as common health-care food and traditional Chinese medicine (TCM), which exerts pharmacological effects, such as anti-cardiovascular, anti-tumor, anti-oxidant, anti-inflammatory, anti-virus, hepatoprotective, blood pressure-lowering and neuroprotective. In this study, reliable, and sensitive ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) methods were developed and validated for the determination of eleven active components in rat plasma after oral administration of the CRP extract. The results of this method exhibited that the specificity, linearity (r > 0.999), precision and accuracy (the coefficient of variation (CV) < 11.5 %), recovery (52.9-107.9 %), matrix effects (63.8-107.5 %), and stability (CV < 10.8 %) met all requirements for the quantitation of plasma samples. The pharmacokinetic results showed that the Tmax of flavone glycosides was less than 0.7 h, and that of polymethoxyflavones and volatile components were within 1-7 h. Meanwhile, the area-under-the-curve (AUC) and concentration maximum (Cmax) of hesperidin, nobiletin, tangeretin, and D-limonene were higher than those of the other components, suggesting that the plasma exposure levels of these constituents were higher in CRP. The present research lays a foundation for elucidating the therapeutic material basis and provides a reference for further scientific research and clinical application of CRP.
摘要：
陈皮（CRP）被用作常见的保健食品和中药（TCM），发挥药理作用，如抗心血管，抗肿瘤，抗氧化剂，抗炎,抗病毒,保肝,血压降低和神经保护。在这项研究中,可靠,建立了灵敏的超高效液相色谱/串联质谱（UPLC-MS/MS）和气相色谱-质谱（GC-MS）方法，并验证了口服给药后大鼠血浆中11种活性成分的含量。CRP提取物。该方法的结果表明，该方法的特异性，线性度（r>0.999），精度和准确度(变异系数(CV)<11.5%)，回收率（52.9-107.9%），基体效应(63.8-107.5%)，和稳定性(CV<10.8%)满足血浆样品定量的所有要求。药代动力学结果表明，黄酮苷的Tmax小于0.7h，多甲氧基黄酮和挥发性成分在1-7小时内。同时，橙皮苷的曲线下面积（AUC）和最大浓度（Cmax），景天苷,橘皮素,D-柠檬烯的含量高于其他组分，提示CRP中这些成分的血浆暴露水平较高。本研究为阐明CRP的治疗物质基础奠定了基础，为CRP的进一步科学研究和临床应用提供了参考。
