Abstract：
The cardiac perivascular niche is a cellular microenvironment of a blood vessel. The principles of niche regulation are still poorly understood. We studied the effect of TGFβ1 on cells forming the cardiac perivascular niche using 3D cell culture (cardiospheres). Cardiospheres contained progenitor (c-Kit), endothelial (CD31), and mural (αSMA) cells, basement membrane proteins (laminin) and extracellular matrix proteins (collagen I, fibronectin). TGFβ1 treatment decreased the length of CD31+ microvasculature, VE cadherin protein level, and proportion of NG2+ cells, and increased proportion of αSMA+ cells and transgelin/SM22α protein level. We supposed that this effect is related to the stabilizing function of TGFβ1 on vascular cells: decreased endothelial cell proliferation, as shown for HUVEC, and activation of mural cell differentiation.
摘要：
心脏血管周围小生境是血管的细胞微环境。生态位调节的原则仍然知之甚少。我们使用3D细胞培养（心球）研究了TGFβ1对形成心脏血管周围小生境的细胞的影响。心球含有祖细胞(c-Kit)，内皮（CD31），和壁（αSMA）细胞，基底膜蛋白质（层粘连蛋白）和细胞外基质蛋白（胶原蛋白I，纤连蛋白）。TGFβ1治疗减少了CD31+微脉管系统的长度，VE钙黏着蛋白水平，和NG2+细胞的比例，αSMA+细胞比例和转胶蛋白/SM22α蛋白水平增加。我们认为这种作用与TGFβ1对血管细胞的稳定功能有关：内皮细胞增殖减少，如HUVEC所示，和壁细胞分化的激活。
