Abstract：
To evaluate the effects of the association of host defence peptide IDR-1002 and ciprofloxacin on human dental pulp cells (hDPSCs). hDPSCs were stimulated with ciprofloxacin and IDR-1002. Cell viability (by MTT assay), migration capacity (by scratch assay), production of inflammatory and anti-inflammatory mediators by hDPSCs (RT-PCR) and osteogenic differentiation (alizarin red staining) were evaluated. Phenotypic profile of hDPSCs demonstrated 97% for positive marked mesenchymal stem cell. Increased pulp cell migration and proliferation were observed after 24 and 48 h of exposure to IDR-1002 with ciprofloxacin. Mineral matrix formation by hDPSCs was observed of the association while its reduction was observed in the presence of peptide. After 24 h, the association between ciprofloxacin and IDR-1002 significantly downregulated TNFRSF-1, IL-1β, IL-8, IL-6 and IL-10 gene expression (p ≤ 0.0001). The association between the IDR-1002 and ciprofloxacin showed favourable immunomodulatory potential, emerging as a promising option for pulp revascularisation processes.
摘要：
评价宿主防御肽IDR-1002与环丙沙星联合对人牙髓细胞(hDPSC)的影响。用环丙沙星和IDR-1002刺激hDPSC。细胞活力（通过MTT测定），迁移能力(通过划痕分析)，通过hDPSC（RT-PCR）和成骨分化（茜素红染色）评估炎症和抗炎介质的产生。hDPSC的表型图谱显示97%的阳性标记间充质干细胞。用环丙沙星暴露于IDR-100224和48小时后，观察到牙髓细胞迁移和增殖增加。观察到由hDPSC形成的矿物基质的缔合,而在肽的存在下观察到其减少。24小时后，环丙沙星和IDR-1002之间的关联显着下调TNFRSF-1，IL-1β，IL-8、IL-6和IL-10基因表达(p≤0.0001)。IDR-1002和环丙沙星之间的关联显示出良好的免疫调节潜力，正在成为牙髓血运重建过程的一种有希望的选择。
