PDF(Pubmed)
Abstract：
The clinical success of implanted biomaterials such as dental implants is largely determined by the molecular signaling that occurs at the tissue-implant interface. The modification of surface topography is a widely-employed strategy for optimizing tissue integration with dental implants. However, little is known regarding the direct, cellular-level effects of substratum topography on platelet signaling and adhesion, despite these cells being the first to encounter the implant surface during surgical placement. Here we compared platelet adhesion and secretion on four (4) different titanium surfaces, notably, the modifications applied to commercially available dental implants: smooth (S) titanium; acid-etched (AE), sandblasted (SB) and a combined acid-etching/sandblasting procedure (SLA). Platelets were isolated from human blood, washed, and seeded on to the 4 test surfaces; platelet adhesion was quantified by microscopy. In addition, the secretion of critical molecules stored in platelet granules (platelet factor 4, PF4; soluble P-selectin, sCD62P; transforming growth factor-beta1, TGF-β1; platelet-derived growth factor-AB, PDGF-AB) was measured by enzyme-linked immunosorbent assay (ELISA) analysis of the supernatants. There was greater platelet adhesion to the rougher AE and SB surfaces, however, the concentration of the secreted growth factors was comparable on all surfaces. We conclude that while surface topography can be engineered to modulate initial platelet adhesion, granule secretion is likely regulated as a separate and independent process.
摘要：
植入的生物材料如牙科植入物的临床成功很大程度上取决于发生在组织-植入物界面处的分子信号传导。表面形貌的修改是用于优化与牙科植入物的组织整合的广泛采用的策略。然而,关于直接的鲜为人知，基质地形图对血小板信号和粘附的细胞水平影响，尽管这些细胞是在手术放置期间第一个遇到植入物表面的细胞。在这里，我们比较了四（4）个不同钛表面上的血小板粘附和分泌，特别是,适用于市售牙科植入物的修改：光滑（S）钛；酸蚀（AE），喷砂（SB）和酸蚀刻/喷砂组合程序（SLA）。血小板是从人体血液中分离出来的,washed,并接种到4个测试表面上；通过显微镜定量血小板粘附。此外,储存在血小板颗粒中的关键分子的分泌(血小板因子4，PF4；可溶性P-选择素，sCD62P;转化生长因子-β1,TGF-β1;血小板源性生长因子-AB,通过上清液的酶联免疫吸附测定（ELISA）分析来测量PDGF-AB）。在粗糙的AE和SB表面上有更大的血小板粘附，然而,分泌的生长因子的浓度在所有表面上是相当的。我们得出的结论是，虽然表面形貌可以设计为调节初始血小板粘附，颗粒分泌可能是作为一个单独和独立的过程进行调节的。
