PDF(Pubmed)
Abstract：
Cleft palate is the most common facial birth defect worldwide. It is caused by environmental factors or genetic mutations. Environmental factors such as pharmaceutical exposure in women are known to induce cleft palate. The aim of the present study was to investigate the protective effect of Sasa veitchii extract against medicine-induced inhibition of proliferation of human embryonic palatal mesenchymal cells. We demonstrated that all-trans-retinoic acid inhibited human embryonic palatal mesenchymal cell proliferation in a dose-dependent manner, whereas dexamethasone treatment had no effect on cell proliferation. Cotreatment with Sasa veitchii extract repressed all-trans-retinoic acid-induced toxicity in human embryonic palatal mesenchymal cells. We found that cotreatment with Sasa veitchii extract protected all-trans-retinoic acid-induced cyclin D1 downregulation in human embryonic palatal mesenchymal cells. Furthermore, Sasa veitchii extract suppressed all-trans-retinoic acid-induced miR-4680-3p expression. Additionally, the expression levels of the genes that function downstream of the target genes ( ERBB2 and JADE1 ) of miR-4680-3p in signaling pathways were enhanced by cotreatment with Sasa veitchii extract and all-trans-retinoic acid compared to all-trans-retinoic acid treatment. These results suggest that Sasa veitchii extract suppresses all-trans-retinoic acid-induced inhibition of cell proliferation via modulation of miR-4680-3p expression.
摘要：
腭裂是全球最常见的面部出生缺陷。它是由环境因素或基因突变引起的。已知环境因素如女性的药物暴露会导致腭裂。本研究的目的是研究Sasaveitchi提取物对药物诱导的人胚pa间充质细胞增殖的保护作用。我们证明了全反式维甲酸以剂量依赖的方式抑制人胚胎腭突间充质细胞增殖,而地塞米松治疗对细胞增殖没有影响。Sasaveitchii提取物的协同治疗抑制了全反式维甲酸诱导的人胚胎腭间质细胞的毒性。我们发现，与Sasaveitchi提取物共治可保护全反式维甲酸诱导的细胞周期蛋白D1在人胚胎pal间充质细胞中的下调。此外，Sasaveitchi提取物抑制全反式维甲酸诱导的miR-4680-3p表达。此外,与全反式视黄酸处理相比,在信号通路中miR-4680-3p的靶基因(ERBB2和JADE1)下游起作用的基因的表达水平通过与Sasaveitchi提取物和全反式视黄酸共同处理得到增强.这些结果表明，Sasaveitchii提取物通过调节miR-4680-3p表达来抑制全反式维甲酸诱导的细胞增殖抑制。
