PDF(Pubmed)
Abstract：
This study provides sequence datasets of endophytic and rhizobacteria of jute using 16S rRNA gene sequencing. The plant samples were first surface sterilized and DNA of the bacteria from soil and jute roots and stem was extracted using Quick-DNA™ Fungal/Bacterial Miniprep Kit. The purified DNA was amplified and subjected to polymerase chain reaction using forward and reverse primers. The PCR products were sequenced on Applied Biosystems ABI 3500XL Genetic Analyser (Applied Biosystems, ThermoFisher Scientific). The sequences were analyzed using BioEdit version 7.2.5 and then BLAST on NCBI. The identifiable bacteria include the rhizobacteria, Citrobacter fruendii RZS23 (accession number: CP024673.1), endophytic bacteria, Bacillus cereus EDR23 (accession number: LN890242.1), and Morganella morganii EDS23 (accession number: KR094121.1). The plant growth-promoting traits exhibited by these bacteria suggest their future exploration as bioinoculants.
摘要：
本研究使用16SrRNA基因测序提供了黄麻内生和根际细菌的序列数据集。首先对植物样品进行表面灭菌，并使用Quick-DNA™真菌/细菌Miniprep试剂盒从土壤和黄麻根和茎中提取细菌的DNA。扩增纯化的DNA并使用正向和反向引物进行聚合酶链式反应。PCR产物在应用生物系统ABI3500XL遗传分析仪（应用生物系统，ThermoFisherScientific)。使用BioEdit版本7.2.5然后在NCBI上BLAST分析序列。可识别的细菌包括根瘤菌，水果柠檬酸杆菌RZS23(登录号:CP024673.1),内生细菌，蜡状芽孢杆菌EDR23(登录号:LN890242.1),和MorganellamorganiiEDS23(登录号:KR094121.1)。这些细菌表现出的植物生长促进特性表明了它们作为生物接种剂的未来探索。
