PDF(Pubmed)
Abstract：
Tea plant (Camellia sinensis [L.]) is one of the most important crops in China, and tea branch is an important agronomic trait that determines the yield of tea plant. In previous work focused on GWAS that detecting GWAS signals related to plant architecture through whole genome re-sequencing of ancient tea plants, a gene locus TEA 029928 significantly related to plant type was found. Sequence alignment results showed that this gene belonged to the F-box family. We named it CsBRC. CsBRC-GFP fusion proteins were mainly localized in the plasma membrane. By comparing the phenotypes of CsBRC transgenic tobacco and WT tobacco, it was found that the number of branches of transgenic tobacco was significantly higher than that of wild-type tobacco. Through RNA-seq analysis, it was found that CsBRC affects the branching development of plants by regulating the expression of genes related to brassinosteroid synthesis pathway in plants. In addition, overexpression of CsBRC in rice could increase tiller number, grain length and width, and 1,000-grain weight.
摘要：
茶树（茶树[L.])是中国最重要的农作物之一，茶枝是决定茶树产量的重要农艺性状。在以前的工作集中在GWAS，通过对古代茶树的全基因组重新测序来检测与植物结构相关的GWAS信号，发现了与植物类型显着相关的TEA029928基因座。序列比对结果表明,该基因属于F-box家族。我们把它命名为CsBRC。CsBRC-GFP融合蛋白主要位于质膜中。通过比较CsBRC转基因烟草和WT烟草的表型,发现转基因烟草的分枝数明显高于野生型烟草。通过RNA-seq分析,研究发现,CsBRC通过调控植物油菜素合成途径相关基因的表达,影响植物的分枝发育。此外,CsBRC在水稻中的过表达可以增加分ill数，谷物长度和宽度，和1000粒的重量。
