PDF(Pubmed)
Abstract：
With worldwide cultivation, the faba bean (Vicia faba L.) stands as one of the most vital cool-season legume crops, serving as a major component of food security. China leads global faba bean production in terms of both total planting area and yield, with major production hubs in Yunnan, Sichuan, Jiangsu, and Gansu provinces. The faba bean viruses have caused serious yield losses in these production areas, but previous researches have not comprehensively investigated this issue. In this study, we collected 287 faba bean samples over three consecutive years from eight provinces/municipalities of China. We employed small RNA sequencing, RT-PCR, DNA sequencing, and phylogenetic analysis to detect the presence of viruses and examine their incidence, distribution, and genetic diversity. We identified a total of nine distinct viruses: bean yellow mosaic virus (BYMV, Potyvirus), milk vetch dwarf virus (MDV, Nanovirus), vicia cryptic virus (VCV, Alphapartitivirus), bean common mosaic virus (BCMV, Potyvirus), beet western yellows virus (BWYV, Polerovirus), broad bean wilt virus (BBWV, Fabavirus), soybean mosaic virus (SMV, Potyvirus), pea seed-borne mosaic virus (PSbMV, Potyvirus), and cucumber mosaic virus (CMV, Cucumovirus). BYMV was the predominant virus found during our sampling, followed by MDV and VCV. This study marks the first reported detection of BCMV in Chinese faba bean fields. Except for several isolates from Gansu and Yunnan provinces, our sequence analysis revealed that the majority of BYMV isolates contain highly conserved nucleotide sequences of coat protein (CP). Amino acid sequence alignment indicates that there is a conserved NAG motif at the N-terminal region of BYMV CP, which is considered important for aphid transmission. Our findings not only highlight the presence and diversity of pathogenic viruses in Chinese faba bean production, but also provide target pathogens for future antiviral resource screening and a basis for antiviral breeding.
摘要：
随着全球种植，蚕豆（ViciafabaL.）是最重要的冷季豆类作物之一，作为粮食安全的重要组成部分。就种植总面积和产量而言，中国领先全球蚕豆产量，在云南设有主要生产中心，四川,江苏,和甘肃省。蚕豆病毒在这些产区造成了严重的产量损失,但是以前的研究并没有全面地研究这个问题。在这项研究中,我们连续三年从中国八个省市收集了287份蚕豆样品。我们采用了小RNA测序，RT-PCR,DNA测序,和系统发育分析以检测病毒的存在并检查其发病率，分布，和遗传多样性。我们总共鉴定了9种不同的病毒：豆黄花叶病毒(BYMV，Potyvirus),vetch矮小病毒(MDV，纳米病毒），蚕豆隐匿病毒(VCV，Alphapartivirus)，豆类常见花叶病毒(BCMV，Potyvirus),甜菜西部黄化病毒(BWYV，Polerovirus),蚕豆枯萎病病毒(BBWV，Fabavirus),大豆花叶病毒(SMV，Potyvirus),豌豆种子传播花叶病毒(PSbMV，Potyvirus),和黄瓜花叶病毒(CMV，Cucumovirus）。BYMV是我们取样时发现的主要病毒,其次是MDV和VCV。这项研究标志着在中国蚕豆田中首次报道了BCMV的检测。除了甘肃和云南的几个分离株，我们的序列分析显示，大多数BYMV分离株含有高度保守的外壳蛋白（CP）核苷酸序列。氨基酸序列比对表明在BYMVCP的N端区域存在保守的NAG基序,这被认为对蚜虫传播很重要。我们的发现不仅突出了中国蚕豆生产中致病病毒的存在和多样性，同时也为今后的抗病毒资源筛选和抗病毒育种提供了依据。
