UNASSIGNED: In this study, we synthesized a dicoumarol-graphene oxide quantum dot (DIC-GQD) polymer with excellent biocompatibility. This polymer was synthesized via an electrostatic adsorption method using the natural drug DIC and GQDs as raw materials.
UNASSIGNED: Our findings demonstrated that DIC exhibits high anti-PRRSV activity by inhibiting the PRRSV replication stage. The transcriptome sequencing analysis revealed that DIC treatment stimulates genes associated with the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signalling pathway. In porcine alveolar macrophages (PAMs), DIC-GQDs induce TYK2, JAK1, STAT1, and STAT2 phosphorylation, leading to the upregulation of JAK1, STAT1, STAT2, interferon-β (IFN-β) and interferon-stimulated genes (ISGs). Animal challenge experiments further confirmed that DIC-GQDs effectively alleviated clinical symptoms and pathological reactions in the lungs, spleen, and lymph nodes of PRRSV-infected pigs.
UNASSIGNED: These findings suggest that DIC-GQDs significantly inhibits PRRSV proliferation by activating the JAK/STAT signalling pathway. Therefore, DIC-GQDs hold promise as an alternative treatment for PRRSV infection.
■在这项研究中,我们合成了具有优异生物相容性的双香豆素-氧化石墨烯量子点(DIC-GQD)聚合物。该聚合物是以天然药物DIC和GQDs为原料,通过静电吸附法合成的。
■我们的发现证明DIC通过抑制PRRSV复制阶段表现出高的抗PRRSV活性。转录组测序分析揭示DIC处理刺激与Janus激酶/信号转导子和转录激活因子(JAK/STAT)信号通路相关的基因。在猪肺泡巨噬细胞(PAMs)中,DIC-GQDs诱导TYK2、JAK1、STAT1和STAT2磷酸化,导致JAK1,STAT1,STAT2,干扰素-β(IFN-β)和干扰素刺激基因(ISG)的上调。动物激发实验进一步证实DIC-GQDs能有效缓解肺部临床症状和病理反应,脾,脾和PRRSV感染猪的淋巴结。
■这些发现表明DIC-GQD通过激活JAK/STAT信号通路显著抑制PRRSV增殖。因此,DIC-GQD有望作为PRRSV感染的替代疗法。