PDF(Pubmed)
Abstract：
Altitude and ultraviolet (UV) radiation may affect the community composition and distribution of microorganisms in soil ecosystems. In this study, 49 soil samples from 10 locations were collected from different elevations on the eastern Pamir Plateau and analyzed for soil microbial community structure and function using high-throughput sequencing. The results showed that soil samples from different elevations of the eastern Pamir Plateau contained 6834 OTUs in 26 phyla and 399 genera. The dominant phyla common to different elevations were Actinobacteria, Proteobacteria, Bacteroidota, Acidobacteriota, and Gemmatimonadota. The dominant genera were Rubrobacter, Sphingomonas, Nocardioides, and Solirubrobacter. Species richness increased slightly with elevation, and there were significant differences in community composition between the elevations. Elevation and UV exposure are important factors that drive changes in bacterial communities. The results of the KEGG pathway showed that drug resistance, antineoplastic, aging, replication, and repair were enhanced and then slightly decreased with increasing elevation. Bacterial communities at different elevations were rich in radiation-resistant microorganisms, and the main genera were Rubrobacter, Sphingomonas, Nocardioides, Pontibacter, and Streptomyces. The findings have shown the composition and distribution of bacterial communities at different elevations on the Eastern Pamir Plateau. Potentially radiation tolerant microbial species were also examined. The results are of considerable importance for the succession of bacterial microorganisms in the plateau region, the study of radiation tolerant bacterial germplasm resources, and the application of biofunctionality.
摘要：
海拔和紫外线（UV）辐射可能会影响土壤生态系统中微生物的群落组成和分布。在这项研究中,收集了帕米尔高原东部10个不同海拔地区的49份土壤样品，采用高通量测序技术对土壤微生物群落结构和功能进行了分析。结果表明，帕米尔高原东部不同海拔的土壤样品在26门和399属中含有6834个OTU。不同海拔常见的优势门是放线菌，变形杆菌,拟杆菌,酸杆菌,还有Gemmatimonadota.主要属为鲁布杆菌属,鞘氨醇单胞菌,诺卡诺德,和Solirubrobacter.物种丰富度随海拔略有增加，海拔高度之间的群落组成存在显着差异。海拔和紫外线暴露是驱动细菌群落变化的重要因素。KEGG通路的结果表明,抗肿瘤,老化，复制,修复增强，然后随海拔升高而略有下降。不同海拔的细菌群落富含抗辐射微生物,主要属是鲁布杆菌属,鞘氨醇单胞菌,诺卡诺德,Pontibacter,和链霉菌.研究结果表明，东帕米尔高原不同海拔的细菌群落组成和分布。还检查了潜在的耐辐射微生物物种。该结果对高原地区细菌微生物的演替具有重要意义,耐辐射细菌种质资源的研究，以及生物功能的应用。
