PDF(Pubmed)
Abstract：
The most serious long-term effects of diabetes is peripheral artery disease (PAD) which increases the chance of developing diabetic foot ulcers, gangrene and even lower limb amputation. The clinical manifestations of PAD which are typically not revealed until symptoms like intermittent claudication, rest pain and ischemic gangrene develop, are not present in majority of diabetes mellitus patients with PAD due to diabetic peripheral neuropathy. Therefore, current study is aimed to evaluate the inflammatory and endothelial dysfunction markers with their correlation to biomarkers that can help for in-time diagnosis and efficient prognosis of developing diabetes-associated PAD. Enzyme-linked immunosorbent assay was used to evaluate the interlukin-6, interlukin-8, intercellular adhesion molecule (ICAM) and vascular cell adhesion molecule (VCAM) in PAD with diabetes group, diabetic group and healthy individual group while biomarkers were measured by kit method. It was observed that serum IL-6, IL-8, ICAM and VCAM levels in type II diabetes mellitus (T2DM) with PAD patients were increased significantly (85.93, 597.08, 94.80 and 80.66) as compared to T2DM patients (59.52, 231.34, 56.88 and 50.19) and healthy individuals (4.81, 16.93, 5.55 and 5.16). The overall means for the parameters, IL-6, IL-8, ICAM, VCAM, urea, S/creatinine, CK-MB, AST, ALT, cholesterol, triglyceride, HDL, LDL, PT, aPTT, INR, HbA1C, and CRP within all groups were significantly (P < 0.05) different from each other. Therefore, it was concluded that the change in IL-6, IL-8, ICAM and VCAM can serve as an accurate diagnostic indicator and successful treatment.
摘要：
糖尿病最严重的长期影响是外周动脉疾病（PAD），这增加了发展糖尿病足溃疡的机会，坏疽甚至下肢截肢。PAD的临床表现通常直到间歇性跛行等症状才被发现，静息疼痛和缺血性坏疽发展，由于糖尿病周围神经病变，在大多数患有PAD的糖尿病患者中不存在。因此,目前的研究旨在评估炎症和内皮功能障碍标志物及其与生物标志物的相关性，这些标志物有助于糖尿病相关PAD的及时诊断和有效预后。采用酶联免疫吸附试验评价糖尿病合并PAD患者的IL-6、IL-8、细胞间黏附分子(ICAM)和血管细胞黏附分子(VCAM),糖尿病组和健康个体组同时采用试剂盒法测量生物标志物。观察到，与T2DM患者（59.52、231.34、56.88和50.19）和健康个体（4.81、16.93、5.55和5.16）相比，患有PAD的II型糖尿病（T2DM）患者的血清IL-6，IL-8，ICAM和VCAM水平显着增加（85.93、597.08、94.80和80.66）。参数的总体平均值，IL-6,IL-8,ICAM,VCAM,尿素,S/肌酐，CK-MB,AST,ALT,胆固醇,甘油三酯，HDL,LDL,PT,aPTT,INR,HbA1C,各组间CRP差异有统计学意义(P<0.05)。因此,结论IL-6，IL-8，ICAM和VCAM的变化可以作为准确的诊断指标和成功的治疗。
