Abstract：
Omega-3 (n3) is a polyunsaturated fatty acid well known for its anti-inflammatory and neuroprotective properties. Obesity is linked to chronic inflammation that disrupts metabolism, the intestine physiology and the central nervous system functioning. This study aims to determine if n3 supplementation can interfere with the effects of obesity on the mitochondrial activity, intestinal barrier, and neurotransmitter levels in the brain of Wistar rats that received cafeteria diet (CAF). We examined adipose tissue, skeletal muscle, plasma, intestine, and the cerebral cortex of four groups: CT (control diet), CTn3 (control diet with n3 supplementation), CAF, and CAFn3 (CAF and n3). Diets were offered for 13 weeks, with n3 supplementation in the final 5 weeks. Adipose tissue Electron Transport Chain complexes I, II, and III showed higher activity in CAF groups, as did complexes III and IV in skeletal muscle. Acetate levels in plasma were reduced in CAF groups, and Lipopolysaccharide (LPS) was higher in the CAF group but reduced in CAFn3 group. Claudin-5 in the intestine was lower in CAF groups, with no n3 supplementation effect. In the cerebral cortex, dopamine levels were decreased with CAF, which was reversed by n3. DOPAC, a dopamine metabolite, also showed a supplementation effect, and HVA, a diet effect. Serotonin levels increased in the CAF group that received supplementation. Therefore, we demonstrate disturbances in mitochondria, plasma, intestine and brain of rats submitted to CAF and the potential benefit of n3 supplementation in endotoxemia and neurotransmitter levels.
摘要：
Omega-3（n3）是一种多不饱和脂肪酸，以其抗炎和神经保护特性而闻名。肥胖与破坏新陈代谢的慢性炎症有关，肠道生理和中枢神经系统功能。这项研究旨在确定补充n3是否可以干扰肥胖对线粒体活性的影响，肠屏障,和接受自助餐（CAF）的Wistar大鼠大脑中的神经递质水平。我们检查了脂肪组织,骨骼肌,等离子体,肠,和四组的大脑皮层：CT（对照饮食），CTn3（补充n3的控制饮食），CAF,和CAFn3（CAF和n3）。饮食提供了13周，在最后5周内补充n3。脂肪组织电子传输链配合物I,II,和III在CAF组中显示出更高的活性，骨骼肌中的复合物III和IV也是如此。CAF组血浆中的醋酸盐水平降低，脂多糖（LPS）在CAF组中较高，而在CAFn3组中较低。CAF组中肠道中的Claudin-5较低，没有n3补充效果。在大脑皮层,多巴胺水平随着CAF降低，被n3逆转了。DOPAC,多巴胺代谢产物,还显示出补充作用，和HVA,一种饮食效果。在接受补充的CAF组中5-羟色胺水平增加。因此,我们证明了线粒体的紊乱，等离子体,接受CAF的大鼠的肠和脑以及补充n3对内毒素血症和神经递质水平的潜在益处。
