Abstract：
OBJECTIVE: Bacterial exopolysaccharides (EPS) possess numerous properties beneficial for the growth of microbes and plants under hostile conditions. The study aimed to develop a bioformulation with bacterial EPS to enhance the bioinoculant\'s shelf life and functional efficacy under salinity stress.
RESULTS: High EPS-producing and salt-tolerant bacterial strain (Bacillus haynessi SD2) exhibiting auxin-production, phosphate-solubilization, and biofilm-forming ability, was selected. EPS-based bioformulation of SD2 improved the growth of three legumes under salt stress, from which pigeonpea was selected for further experiments. SD2 improved the growth and lowered the accumulation of stress markers in plants under salt stress. Bioformulations with varying EPS concentrations (1% and 2%) were stored for 6 months at 4°C, 30°C, and 37°C to assess their shelf life and functional efficacy. The shelf life and efficacy of EPS-based bioformulation were sustained even after 6 months of storage at high temperature, enhancing pigeonpea growth under stress in both control and natural conditions. However, the efficacy of non EPS-based bioformulation declined following four months of storage. The bioformulation (with 1% EPS) modulated bacterial abundance in the plant\'s rhizosphere under stress conditions.
CONCLUSIONS: The study brings forth a new strategy for developing next-generation bioformulations with higher shelf life and efficacy for salinity stress management in pigeonpea.
RESULTS: High EPS-producing and salt-tolerant bacterial strain (Bacillus haynessi SD2) exhibiting auxin-production, phosphate-solubilization, and biofilm-forming ability, was selected. EPS-based bioformulation of SD2 improved the growth of three legumes under salt stress, from which pigeonpea was selected for further experiments. SD2 improved the growth and lowered the accumulation of stress markers in plants under salt stress. Bioformulations with varying EPS concentrations (1% and 2%) were stored for 6 months at 4°C, 30°C, and 37°C to assess their shelf life and functional efficacy. The shelf life and efficacy of EPS-based bioformulation were sustained even after 6 months of storage at high temperature, enhancing pigeonpea growth under stress in both control and natural conditions. However, the efficacy of non EPS-based bioformulation declined following four months of storage. The bioformulation (with 1% EPS) modulated bacterial abundance in the plant\'s rhizosphere under stress conditions.
CONCLUSIONS: The study brings forth a new strategy for developing next-generation bioformulations with higher shelf life and efficacy for salinity stress management in pigeonpea.
摘要：
目的：细菌胞外多糖（EPS）具有许多有利于微生物和植物在恶劣条件下生长的特性。该研究旨在开发具有细菌EPS的生物制剂，以提高生物接种剂在盐度胁迫下的保质期和功能功效。
结果：表现出生长素生产的高EPS和耐盐细菌菌株（SD2），磷酸盐溶解，并选择了生物膜形成能力。基于EPS的SD2生物配方改善了盐胁迫下三种豆科植物的生长，从中选择木豆进行进一步的实验。SD2改善了盐胁迫下植物的生长并降低了胁迫标记的积累。将具有不同EPS浓度（1％和2％）的生物制剂在4°C下储存6个月，30°C,和37°C以评估其保质期和功能功效。基于EPS的生物制剂的保质期和功效在较高的温度下持续，在对照和自然条件下储存六个月后，在胁迫下促进木豆的生长。然而,非基于EPS的生物制剂的功效在储存四个月后下降.在胁迫条件下，生物制剂调节了植物根际的细菌丰度。
■该研究提出了一种开发下一代生物制剂的新策略，该制剂具有更高的保质期和在盐水条件下对木豆进行盐度应激管理的功效。
结果：表现出生长素生产的高EPS和耐盐细菌菌株（SD2），磷酸盐溶解，并选择了生物膜形成能力。基于EPS的SD2生物配方改善了盐胁迫下三种豆科植物的生长，从中选择木豆进行进一步的实验。SD2改善了盐胁迫下植物的生长并降低了胁迫标记的积累。将具有不同EPS浓度（1％和2％）的生物制剂在4°C下储存6个月，30°C,和37°C以评估其保质期和功能功效。基于EPS的生物制剂的保质期和功效在较高的温度下持续，在对照和自然条件下储存六个月后，在胁迫下促进木豆的生长。然而,非基于EPS的生物制剂的功效在储存四个月后下降.在胁迫条件下，生物制剂调节了植物根际的细菌丰度。
■该研究提出了一种开发下一代生物制剂的新策略，该制剂具有更高的保质期和在盐水条件下对木豆进行盐度应激管理的功效。
