Abstract：
Deoxynivalenol (DON) is widely found in food and feed, posing a threat to human and animal health. Lycopene (Lyc) is a natural plant extracts with significant antioxidant properties. This study was conducted to investigate the protective effects of Lyc on IPEC-J2 cells upon DON exposure. The detection of cell viability and trypan blue staining showed that Lyc alleviated cell damage and decreased cell apoptotic rate induced by DON. The analysis of reactive oxygen species (ROS) level and antioxidant parameter measurements showed that Lyc significantly down-regulated the content of ROS and restored antioxidant enzyme activity. Furthermore, mitochondrial membrane potential (ΔΨm) detection, mitochondrial DNA copy number (mtDNAcn) assay and adenosine triphosphate (ATP) concentration detection showed Lyc improved mitochondrial function after DON exposure. The results of transcriptome analysis, ROS detection and CCK8 assay suggested that Lyc may activated the oxidative phosphorylation (OXPHOS) to improve mitochondrial function. Conclusively, our results suggested that Lyc alleviated DON-induced oxidative stress by improving mitochondrial function through OXPHOS signaling pathway.
摘要：
脱氧雪腐镰刀菌烯醇(DON)广泛存在于食品和饲料中,对人类和动物健康构成威胁。番茄红素(Lyc)是一种具有显著抗氧化特性的天然植物提取物。本研究旨在研究Lyc对DON暴露后IPEC-J2细胞的保护作用。细胞活力和锥虫蓝染色检测表明，Lyc减轻了DON诱导的细胞损伤，降低了细胞凋亡率。对活性氧（ROS）水平和抗氧化参数测量的分析表明，Lyc显着下调了ROS的含量并恢复了抗氧化酶的活性。此外，线粒体膜电位(ΔkW)检测，线粒体DNA拷贝数（mtDNAcn）测定和三磷酸腺苷（ATP）浓度检测显示Lyc改善了DON暴露后的线粒体功能。转录组分析的结果，ROS检测和CCK8实验表明Lyc可能激活氧化磷酸化（OXPHOS）以改善线粒体功能。最后，结果提示Lyc通过OXPHOS信号通路改善线粒体功能,减轻DON诱导的氧化应激.
