Abstract:
New mixtures of pesticides are being placed on the market to increase the spectrum of phytosanitary action. Thus, the eco(geno)toxic effects of the new commercial mixture named Platinum Neo, as well as its constituents the neonicotinoid Thiamethoxam and the pyrethroid Lambda-Cyhalothrin, were investigated using the species Daphnia magna, Raphidocelis subcapitata, Danio rerio, and Allium cepa L. The lowest- and no-observed effect concentration (LOEC and NOEC) were measured in ecotoxicological tests. While Thiamethoxam was ecotoxic at ppm level, Lambda-Cyhalothrin and Platinum Neo formulation were ecotoxic at ppb level. The mitotic index (MI), chromosomal aberrations and micronucleus [MN] frequency were measured as indicators of phytogenotoxicity in A. cepa plants exposed for 12 hours to the different insecticides and their mixture under different dilutions. There were significant alterations in the MI and MN frequency in comparison with the A. cepa negative control group, with Thiamethoxam, Lambda-Cyhalothrin, and Platinum Neo treatments all significantly reducing MI and increasing MN frequency. Thus, MI reduction was found at 13.7 mg L-1 for Thiamethoxam, 0.8 μg L-1 for Lambda-Cyahalothrin, and 2.7:2 μg L-1 for Platinum Neo, while MN induction was not observed at 14 mg L-1 for Thiamethoxam, 0.8 μg L-1 for Lambda-Cyahalothrin, and 1.4:1 μg L-1 for Platinum Neo. The insecticide eco(geno)toxicity hierarchy was Platinun Neo > Lambda-Cyhalothrin > Thiamethoxam, and the organism sensitivity hierarchy was daphnids > fish > algae >A. cepa. Eco(geno)toxicity studies of new pesticide mixtures can be useful for management, risk assessment, and avoiding impacts of these products on living beings.
摘要:
新的农药混合物正在投放市场,以增加植物检疫作用的范围。因此,名为PlatinumNeo的新商业混合物的生态(Geno)毒性作用,以及其成分新烟碱噻虫嗪和拟除虫菊酯Lambda-氯氟氰菊酯,使用大型水蚤物种进行了调查,头骨下,DanioRerio,在生态毒理学测试中测量了最低和未观察到的效应浓度(LOEC和NOEC)。虽然噻虫嗪在ppm水平具有生态毒性,λ-氯氟氰菊酯和铂新制剂在ppb水平具有生态毒性。有丝分裂指数(MI),测量了染色体畸变和微核[MN]频率,作为在不同稀释度下暴露于不同杀虫剂及其混合物12小时的安排植物的植物遗传毒性的指标。与A.cepa阴性对照组相比,MI和MN频率有显著变化,用噻虫嗪,λ-氯氟氰菊酯,和PlatinumNeo治疗均显着减少MI并增加MN频率。因此,噻虫嗪在13.7mgL-1时发现MI减少,0.8μgL-1表示λ-氯氟氰菊酯,和2.7:铂金Neo的2μgL-1,而噻虫嗪在14mgL-1时未观察到MN诱导,0.8μgL-1表示λ-氯氟氰菊酯,和1.4:1μgL-1的铂金Neo。杀虫剂生态(基因)毒性等级为PlatinunNeo>Lambda-氯氟氰菊酯>噻虫嗪,生物敏感性等级为水蚤>鱼>藻类>A.cepa.新农药混合物的生态(基因)毒性研究可用于管理,风险评估,并避免这些产品对生物的影响。