Abstract：
BACKGROUND: Platelet plays a vital role in both physiological and pathological processes. However, the limited storage time of platelet in vitro poses an immense challenge for its applications because of the increased risk of bacterial contamination and platelet storage lesions. Agitation can inhibit lesions by facilitating continuous oxygenation of platelets and permitting excess carbon dioxide to be removed during storage. However, it is still not known whether agitating BCs gives a positive effect on platelet quality.
OBJECTIVE: To evaluate the quality difference between platelet concentrates (PCs) from buffy coats (BCs) held rest and agitation.
METHODS: Samples were withdrawn for cell count, blood gas analysis, free hemoglobin level, hypotonic shock response, maximum aggregation rate, activation marker expression (CD62P and CD42b) and coagulation function.
RESULTS: We found the PCs prepared from the agitating BCs had fewer residual WBCs, exhibited a better gas exchange ability, slower metabolism (higher pH, higher content glucose, and lower lactic acid levels), better hypotonic shock response, and lower levels of CD62P. The TEG-PC assays showed no difference in coagulation function.
CONCLUSIONS: Our findings showed that BC can be agitated overnight before a soft spin.
OBJECTIVE: To evaluate the quality difference between platelet concentrates (PCs) from buffy coats (BCs) held rest and agitation.
METHODS: Samples were withdrawn for cell count, blood gas analysis, free hemoglobin level, hypotonic shock response, maximum aggregation rate, activation marker expression (CD62P and CD42b) and coagulation function.
RESULTS: We found the PCs prepared from the agitating BCs had fewer residual WBCs, exhibited a better gas exchange ability, slower metabolism (higher pH, higher content glucose, and lower lactic acid levels), better hypotonic shock response, and lower levels of CD62P. The TEG-PC assays showed no difference in coagulation function.
CONCLUSIONS: Our findings showed that BC can be agitated overnight before a soft spin.
摘要：
背景：血小板在生理和病理过程中起着至关重要的作用。然而,由于细菌污染和血小板储存损害的风险增加，血小板在体外的储存时间有限对其应用提出了巨大挑战。搅动可以通过促进血小板的连续氧合并允许在储存期间除去过量的二氧化碳来抑制损伤。然而,目前尚不清楚搅拌BCs是否会对血小板质量产生积极影响。
目的：评估来自血沉棕黄层（BC）的血小板浓缩物（PC）之间的质量差异。
方法：取出样品进行细胞计数，血气分析,游离血红蛋白水平，低渗休克反应，最大聚合速率,活化标记物表达(CD62P和CD42b)和凝血功能。
结果：我们发现由搅拌的BCs制备的PC具有较少的残留WBC，表现出更好的气体交换能力，较慢的新陈代谢(较高的pH，葡萄糖含量较高，和较低的乳酸水平)，更好的低渗休克反应，和较低水平的CD62P。TEG-PC测定显示凝血功能无差异。
结论：我们的研究结果表明，BC可以在软旋转之前搅拌过夜。
目的：评估来自血沉棕黄层（BC）的血小板浓缩物（PC）之间的质量差异。
方法：取出样品进行细胞计数，血气分析,游离血红蛋白水平，低渗休克反应，最大聚合速率,活化标记物表达(CD62P和CD42b)和凝血功能。
结果：我们发现由搅拌的BCs制备的PC具有较少的残留WBC，表现出更好的气体交换能力，较慢的新陈代谢(较高的pH，葡萄糖含量较高，和较低的乳酸水平)，更好的低渗休克反应，和较低水平的CD62P。TEG-PC测定显示凝血功能无差异。
结论：我们的研究结果表明，BC可以在软旋转之前搅拌过夜。
