Abstract：
HIV-1 is characterized by remarkable genetic diversity resulting from its high replication rate, error-prone reverse transcriptase enzyme and recombination events. In Uganda, HIV-1 subtype diversity is mostly dominated by subtypes A, D, and A1/D Unique Recombinant Forms (URFs). In this study, deep sequences of HIV from patients with known antiretroviral therapy (ART) status were analyzed to determine the subtypes and to identify drug-resistance mutations circulating in the study population. Of the 187 participant samples processed for next-generation sequencing (NGS), 137 (73%) were successfully classified. The majority of HIV-1 strains were classified as subtype A (75, 55%), D (43, 31%), with other subtypes including C (3, 2%), A1/D (9, 7%) and CRF10_CD (1, <1%). Recombinant analysis of nine complete A1/D HIV genomes identified novel recombination patterns described herein. Furthermore, we report for the first time in Uganda, an HIV-1 CRF10_CD strain from a fisherfolk in a Lake Victoria Island fishing community.
摘要：
HIV-1的特点是由于其高复制率导致了显著的遗传多样性，易错的逆转录酶和重组事件。在乌干达,HIV-1亚型的多样性主要以A亚型为主,D,和A1/D独特重组形式(URFs)。在这项研究中,我们对已知抗逆转录病毒治疗(ART)状态的患者的HIV深层序列进行了分析,以确定亚型,并确定研究人群中流行的耐药突变.在为下一代测序（NGS）处理的187个参与者样本中，137（73%）被成功分类。大多数HIV-1病毒株被归类为A型亚型（75，55％），D（43，31%），与其他亚型，包括C（3，2％），A1/D(9，7%)和CRF10_CD(1，<1%)。9个完整A1/DHIV基因组的重组分析鉴定了本文所述的新重组模式。此外，我们第一次在乌干达报道，来自维多利亚湖岛捕鱼社区的渔民的HIV-1CRF10_CD菌株。
