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Abstract：
UNASSIGNED: The purpose of this study was to investigate the presence of sex-steroid receptors in human choroidal tissue across different ages and sex, aiming to better understand the pronounced sex difference in central serous chorioretinopathy (CSC) occurrence.
UNASSIGNED: Paraffin-embedded enucleated eyes of 14 premenopausal women, 15 postmenopausal women, 10 young men (<45 years), and 10 older men (>60 years) were used. A clinically certified immunostaining was performed to detect the presence of the androgen receptor (AR), progesterone receptor (PR; isoform A and B), and estrogen receptor (ERα). The stained slides were scored in a blinded manner for positive endothelial cells and stromal cells in consecutive sections of the same choroidal region.
UNASSIGNED: Our analysis revealed the presence of AR, PR, and ERα in endothelial cells and stromal cells of choroidal tissue. The mean proportion of AR-positive endothelial cells was higher in young men (46% ± 0.15) compared to aged-matched women (29% ± 0.12; P < 0.05, 95% confidence interval [CI]). Premenopausal women showed markedly lower mean proportion of ERα (5% ± 0.02) and PR-positive endothelial cells (2% ± 0.01) compared to postmenopausal women (15% ± 0.07 and 19% ± 0.13; both P < 0.05, 95% CI), young men (13% ± 0.04 and 21% ± 0.10; both P < 0.05, 95% CI), and older men (18% ± 0.09 and 27% ± 0.14; both P < 0.05, 95% CI). Mean PR-positive stromal cells were also less present in premenopausal women (12% ± 0.07) than in other groups.
UNASSIGNED: The number of sex-steroid receptors in the choroidal tissue differs between men and women across different ages, which aligns with the prevalence patterns of CSC in men and postmenopausal women.
UNASSIGNED: Paraffin-embedded enucleated eyes of 14 premenopausal women, 15 postmenopausal women, 10 young men (<45 years), and 10 older men (>60 years) were used. A clinically certified immunostaining was performed to detect the presence of the androgen receptor (AR), progesterone receptor (PR; isoform A and B), and estrogen receptor (ERα). The stained slides were scored in a blinded manner for positive endothelial cells and stromal cells in consecutive sections of the same choroidal region.
UNASSIGNED: Our analysis revealed the presence of AR, PR, and ERα in endothelial cells and stromal cells of choroidal tissue. The mean proportion of AR-positive endothelial cells was higher in young men (46% ± 0.15) compared to aged-matched women (29% ± 0.12; P < 0.05, 95% confidence interval [CI]). Premenopausal women showed markedly lower mean proportion of ERα (5% ± 0.02) and PR-positive endothelial cells (2% ± 0.01) compared to postmenopausal women (15% ± 0.07 and 19% ± 0.13; both P < 0.05, 95% CI), young men (13% ± 0.04 and 21% ± 0.10; both P < 0.05, 95% CI), and older men (18% ± 0.09 and 27% ± 0.14; both P < 0.05, 95% CI). Mean PR-positive stromal cells were also less present in premenopausal women (12% ± 0.07) than in other groups.
UNASSIGNED: The number of sex-steroid receptors in the choroidal tissue differs between men and women across different ages, which aligns with the prevalence patterns of CSC in men and postmenopausal women.
摘要：
■这项研究的目的是调查不同年龄和性别的人脉络膜组织中性激素受体的存在，旨在更好地了解中心性浆液性脉络膜视网膜病变（CSC）发生的明显性别差异。
■14名绝经前妇女的石蜡包埋摘除眼睛，15名绝经后妇女,10名年轻男子（<45岁），使用了10名年龄较大的男性（>60岁）。进行临床认证的免疫染色以检测雄激素受体（AR）的存在，孕激素受体（PR；亚型A和B），和雌激素受体（ERα）。对于相同脉络膜区域的连续切片中的阳性内皮细胞和基质细胞,以盲法方式对染色的载玻片进行评分。
■我们的分析揭示了AR的存在，PR,和血管内皮细胞和脉络膜组织基质细胞中的ERα。年轻男性AR阳性内皮细胞的平均比例（46％±0.15）高于老年女性（29％±0.12；P<0.05，95％置信区间[CI]）。绝经前妇女的ERα（5％±0.02）和PR阳性内皮细胞（2％±0.01）的平均比例明显低于绝经后妇女（15％±0.07和19％±0.13；P<0.05，95％CI），年轻男性（13%±0.04和21%±0.10；两者P<0.05，95%CI），和老年男性（18％±0.09和27％±0.14;P<0.05，95％CI）。与其他组相比，绝经前妇女的平均PR阳性基质细胞也较少（12％±0.07）。
■脉络膜组织中的性类固醇受体的数量在不同年龄的男性和女性之间有所不同，这与男性和绝经后女性的CSC流行模式一致。
■14名绝经前妇女的石蜡包埋摘除眼睛，15名绝经后妇女,10名年轻男子（<45岁），使用了10名年龄较大的男性（>60岁）。进行临床认证的免疫染色以检测雄激素受体（AR）的存在，孕激素受体（PR；亚型A和B），和雌激素受体（ERα）。对于相同脉络膜区域的连续切片中的阳性内皮细胞和基质细胞,以盲法方式对染色的载玻片进行评分。
■我们的分析揭示了AR的存在，PR,和血管内皮细胞和脉络膜组织基质细胞中的ERα。年轻男性AR阳性内皮细胞的平均比例（46％±0.15）高于老年女性（29％±0.12；P<0.05，95％置信区间[CI]）。绝经前妇女的ERα（5％±0.02）和PR阳性内皮细胞（2％±0.01）的平均比例明显低于绝经后妇女（15％±0.07和19％±0.13；P<0.05，95％CI），年轻男性（13%±0.04和21%±0.10；两者P<0.05，95%CI），和老年男性（18％±0.09和27％±0.14;P<0.05，95％CI）。与其他组相比，绝经前妇女的平均PR阳性基质细胞也较少（12％±0.07）。
■脉络膜组织中的性类固醇受体的数量在不同年龄的男性和女性之间有所不同，这与男性和绝经后女性的CSC流行模式一致。
