PDF(Pubmed)
Abstract：
UNASSIGNED: The purpose of this study was to evaluate the biomechanical and hydration differences in scleral tissue after two modalities of collagen cross-linking.
UNASSIGNED: Scleral tissue from 40 adult white rabbit eyes was crosslinked by application of 0.1% Rose Bengal solution followed by 80 J/cm2 green light irradiation (RGX) or by application of 0.1% riboflavin solution followed by 5.4 J/cm2 ultraviolet A irradiation (UVX). Posterior scleral strips were excised from treated and untreated sclera for tensile and hydration-tensile tests. For tensile tests, the strips were subjected to uniaxial extension after excision. For hydration-tensile tests, the strips were dehydrated, rehydrated, and then tested. Young\'s modulus at 8% strain and swelling rate were estimated. ANOVAs were used to test treated-induced differences in scleral biomechanical and hydration properties.
UNASSIGNED: Photo-crosslinked sclera tissue was stiffer (Young\'s modulus at 8% strain: 10.7 ± 4.5 MPa, on average across treatments) than untreated scleral tissue (7.1 ± 4.0 MPa). Scleral stiffness increased 132% after RGX and 90% after UVX compared to untreated sclera. Scleral swelling rate was reduced by 11% after RGX and by 13% after UVX. The stiffness of the treated sclera was also associated with the tissue hydration level. The lower the swelling, the higher the Young\'s modulus of RGX (-3.8% swelling/MPa) and UVX (-3.5% swelling/MPa) treated sclera.
UNASSIGNED: Cross-linking with RGX and UVX impacted the stiffness and hydration of rabbit posterior sclera. The Rose Bengal with green light irradiation may be an alternative method to determine the efficacy and suitability of inducing scleral tissue stiffening in the treatment of myopia.
UNASSIGNED: Scleral tissue from 40 adult white rabbit eyes was crosslinked by application of 0.1% Rose Bengal solution followed by 80 J/cm2 green light irradiation (RGX) or by application of 0.1% riboflavin solution followed by 5.4 J/cm2 ultraviolet A irradiation (UVX). Posterior scleral strips were excised from treated and untreated sclera for tensile and hydration-tensile tests. For tensile tests, the strips were subjected to uniaxial extension after excision. For hydration-tensile tests, the strips were dehydrated, rehydrated, and then tested. Young\'s modulus at 8% strain and swelling rate were estimated. ANOVAs were used to test treated-induced differences in scleral biomechanical and hydration properties.
UNASSIGNED: Photo-crosslinked sclera tissue was stiffer (Young\'s modulus at 8% strain: 10.7 ± 4.5 MPa, on average across treatments) than untreated scleral tissue (7.1 ± 4.0 MPa). Scleral stiffness increased 132% after RGX and 90% after UVX compared to untreated sclera. Scleral swelling rate was reduced by 11% after RGX and by 13% after UVX. The stiffness of the treated sclera was also associated with the tissue hydration level. The lower the swelling, the higher the Young\'s modulus of RGX (-3.8% swelling/MPa) and UVX (-3.5% swelling/MPa) treated sclera.
UNASSIGNED: Cross-linking with RGX and UVX impacted the stiffness and hydration of rabbit posterior sclera. The Rose Bengal with green light irradiation may be an alternative method to determine the efficacy and suitability of inducing scleral tissue stiffening in the treatment of myopia.
摘要：
■这项研究的目的是评估两种胶原蛋白交联方式后巩膜组织的生物力学和水合差异。
■通过应用0.1％玫瑰红溶液，然后进行80J/cm2绿光照射（RGX）或应用0.1％核黄素溶液，然后进行5.4J/cm2紫外线A照射（UVX），使40只成年白兔眼的巩膜组织交联。从经治疗和未经治疗的巩膜上切下后巩膜条进行拉伸和水合拉伸试验。对于拉伸试验，在切除后对条带进行单轴延伸。对于水化拉伸试验，条被脱水了，再水合,然后测试。估计了在8%应变下的杨氏模量和溶胀率。ANOVAs用于测试治疗诱导的巩膜生物力学和水合性能的差异。
■光交联巩膜组织较硬（8%应变时的杨氏模量：10.7±4.5MPa，治疗期间的平均值）比未经治疗的巩膜组织（7.1±4.0MPa）。与未处理的巩膜相比,RGX后巩膜硬度增加132%,UVX后增加90%。RGX后巩膜肿胀率降低了11%，UVX后降低了13%。经处理的巩膜的硬度也与组织水合水平相关。肿胀越低，RGX(-3.8%溶胀/MPa)和UVX(-3.5%溶胀/MPa)处理的巩膜的杨氏模量越高。
■与RGX和UVX的交联影响了兔后巩膜的硬度和水合作用。绿光照射的玫瑰红可能是确定在近视治疗中诱导巩膜组织硬化的功效和适用性的替代方法。
■通过应用0.1％玫瑰红溶液，然后进行80J/cm2绿光照射（RGX）或应用0.1％核黄素溶液，然后进行5.4J/cm2紫外线A照射（UVX），使40只成年白兔眼的巩膜组织交联。从经治疗和未经治疗的巩膜上切下后巩膜条进行拉伸和水合拉伸试验。对于拉伸试验，在切除后对条带进行单轴延伸。对于水化拉伸试验，条被脱水了，再水合,然后测试。估计了在8%应变下的杨氏模量和溶胀率。ANOVAs用于测试治疗诱导的巩膜生物力学和水合性能的差异。
■光交联巩膜组织较硬（8%应变时的杨氏模量：10.7±4.5MPa，治疗期间的平均值）比未经治疗的巩膜组织（7.1±4.0MPa）。与未处理的巩膜相比,RGX后巩膜硬度增加132%,UVX后增加90%。RGX后巩膜肿胀率降低了11%，UVX后降低了13%。经处理的巩膜的硬度也与组织水合水平相关。肿胀越低，RGX(-3.8%溶胀/MPa)和UVX(-3.5%溶胀/MPa)处理的巩膜的杨氏模量越高。
■与RGX和UVX的交联影响了兔后巩膜的硬度和水合作用。绿光照射的玫瑰红可能是确定在近视治疗中诱导巩膜组织硬化的功效和适用性的替代方法。
