Abstract：
Carnobacterium maltaromaticum is a species of lactic acid bacteria (LAB) that has been isolated from various natural environments. It is well-known for producing a diverse spectrum of bacteriocins with potential biotechnological applications. In the present study, a new psychrotolerant strain of C. maltaromaticum CM22 is reported, isolated from a salmon gut sample and producing a variant of the bacteriocin piscicolin 126 that has been named piscicolin CM22. After identification by 16S rRNA gene, this strain has been genomically characterized by sequencing and assembling its complete genome. Moreover, its bacteriocin was purified and characterized. In vitro tests demonstrated that both the strain and its bacteriocin possess antimicrobial activity against several Gram-positive bacteria of interest in human and animal health, such as Listeria monocytogenes, Clostridium perfringens, or Enterococcus faecalis. However, this bacteriocin did not produce any antimicrobial effect on Gram-negative species. The study of its genome showed the genetic structure of the gene cluster that encodes the bacteriocin, showing a high degree of homology to the gene cluster of piscicolin 126 described in other C. maltaromaticum. Although more studies are necessary concerning its functional properties, this new psychrotolerant strain C. maltaromaticum CM22 and its bacteriocin could be considered an interesting candidate with potential application in agri-food industry.
摘要：
Maltaromaticum是一种乳酸菌（LAB），已从各种自然环境中分离出来。众所周知，它可以生产具有潜在生物技术应用的多种细菌素。在本研究中,报道了一种新的精神耐受品系。阳草CM22，从鲑鱼肠道样品中分离，并产生细菌素piscicolin126的变体，该变体已被命名为piscicolinCM22。经16SrRNA基因鉴定，该菌株已通过测序和组装其完整基因组进行了基因组表征。此外，对其细菌素进行了纯化和鉴定。体外试验表明，该菌株及其细菌素对人类和动物健康中的几种革兰氏阳性细菌均具有抗菌活性，比如单核细胞增生李斯特菌，产气荚膜梭菌,或粪肠球菌。然而,这种细菌素对革兰氏阴性细菌没有产生任何抗菌作用.对其基因组的研究显示了编码细菌素的基因簇的遗传结构，显示与其他拟南芥中描述的piscicolin126的基因簇具有高度同源性。尽管需要对其功能特性进行更多的研究，这种新的精神耐受性菌株C.malaromaticumCM22及其细菌素可以被认为是在农业食品工业中具有潜在应用的有趣候选者。
