Abstract：
Cordyceps cicadae is recognized for its medicinal properties, attributed to bioactive constituents like polysaccharides and adenosine, which have been shown to improve kidney and liver functions and possess anti-tumor properties. Rho GTPase activating proteins (Rho GAPs) serve as inhibitory regulators of Rho GTPases in eukaryotic cells by accelerating the GTP hydrolysis of Rho GTPases, leading to their inactivation. In this study, we explored the function of the CcRga8 gene in C. cicadae, which encodes a Rho-type GTPase activating protein. Our study found that the knockout of CcRga8 resulted in a decrease in polysaccharide levels and an increase in adenosine concentration. Furthermore, the mutants exhibited altered spore yield and morphology, fruiting body development, decreased infectivity, reduced resistance to hyperosmotic stress, oxidative conditions, and cell wall inhibitors. These findings suggest that CcRga8 plays a crucial role in the development, stress response, and bioactive compound production of C. cicadae.
摘要：
冬虫夏草因其药用特性而被认可，归因于生物活性成分，如多糖和腺苷，已被证明可以改善肾脏和肝脏功能并具有抗肿瘤特性。RhoGTPase激活蛋白（RhoGAP）通过加速RhoGTPases的GTP水解，在真核细胞中充当RhoGTPases的抑制调节剂，导致它们失活。在这项研究中,我们探索了CcRga8基因在蝉中的功能，其编码Rho型GTPase激活蛋白。我们的研究发现，敲除CcRga8导致多糖水平降低和腺苷浓度增加。此外，突变体表现出改变的孢子产量和形态，子实体发育，传染性降低，对高渗胁迫的抵抗力降低，氧化条件，和细胞壁抑制剂。这些发现表明，CcRga8在发育中起着至关重要的作用，应激反应，和菊科的生物活性化合物生产。
