PDF(Pubmed)
Abstract：
Endophytes play an important role in plant development, survival, and establishment, but their temporal dynamics in young conifer plants are still largely unknown. In this study, the bacterial community was determined by metabarcoding of the 16S rRNA gene in the rhizoplane, roots, and aerial parts of 1- and 5-month-old seedlings of natural populations of Abies religiosa (Kunth) Schltdl. & Cham. In 1-month-old seedlings, Pseudomonas dominated aerial parts (relative abundance 71.6%) and roots (37.9%). However, the roots exhibited significantly higher bacterial species richness than the aerial parts, with the dissimilarity between these plant sections mostly explained by the loss of bacterial amplification sequence variants. After 5 months, Mucilaginibacter dominated in the rhizoplane (9.0%), Streptomyces in the roots (12.2%), and Pseudomonas in the aerial parts (18.1%). The bacterial richness and community structure differed significantly between the plant sections, and these variations were explained mostly by 1-for-1 substitution. The relative abundance of putative metabolic pathways significantly differed between the plant sections at both 1 and 5 months. All the dominant bacterial genera (e.g., Pseudomonas and Burkholderia-Caballeronia-Paraburkholderia) have been reported to have plant growth-promoting capacities and/or antagonism against pathogens, but what defines their role for plant development has still to be determined. This investigation improves our understanding of the early plant-bacteria interactions essential for natural regeneration of A. religiosa forest.
摘要：
内生真菌在植物发育中起着重要的作用,生存,和建立，但是它们在幼小针叶树植物中的时间动态仍然未知。在这项研究中,细菌群落是通过根际平面中16SrRNA基因的元编码确定的，根,冷杉（Kunth）Schltdl自然种群的1个月和5个月大幼苗的地上部分。&Cham。在1个月大的幼苗中，假单胞菌占地上部分（相对丰度71.6％）和根（37.9％）。然而,根表现出明显高于地上部分的细菌物种丰富度，这些植物部分之间的差异主要由细菌扩增序列变体的丢失来解释。5个月后,在根际平面中占主导地位的粘菌肽（9.0％），根中的链霉菌（12.2%），和地上部分的假单胞菌(18.1%)。植物切片之间的细菌丰富度和群落结构差异显著，这些变化主要通过1对1替换来解释。在1个月和5个月的植物切片之间，推定的代谢途径的相对丰度显着不同。所有优势细菌属(例如，据报道，假单胞菌和伯克霍尔德氏菌-卡波隆氏菌-Paraburkholderia）具有促进植物生长的能力和/或对病原体的拮抗作用，但是如何定义它们在植物发育中的作用仍有待确定。这项调查提高了我们对A.religiosa森林自然再生所必需的早期植物-细菌相互作用的理解。
