Abstract：
A novel slightly halophilic, aerobic, and Gram-stain-negative strain, designated as CH-27T, was isolated during a bacterial resource investigation of intertidal sediment collected from Xiaoshi Island in Weihai, PR China. Cells of strain CH-27T were rod-shaped with widths of 0.3-0.6 µm and lengths of 2.0-11.0 µm. Strain CH-27T grew optimally at 37 °C, pH 7.0 and with 2.0 % (w/v) NaCl. Catalase activity was weakly positive and oxidase activity was positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CH-27T was most related to Marinihelvus fidelis KCTC 92639T (93.6 %), followed by Wenzhouxiangella marina MCCC 1K00261T (92.0 %). Based on genome comparisons between strain CH-27T and M. fidelis KCTC 92639T, the average amino acid identity was 63.6 % and the percentage of conserved proteins was 48.3 %. The major cellular fatty acid of strain CH-27T (≥10 %) was iso-C15 : 0 and the sole respiratory quinone was quinone-8. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, and aminophospholipid. The DNA G+C content was 62.7 mol%. Based on comprehensive analysis of its phylogenetic, physiological, biochemical, and chemotaxonomic characteristics, strain CH-27T represents a novel species in a novel genus, for which the name Elongatibacter sediminis gen. nov., sp.nov. is proposed. The type strain is CH-27T (=MCCC 1H00480T=KCTC 8011T).
摘要：
一部小说，轻微的嗜盐，有氧,和革兰氏染色阴性菌株，指定为CH-27T，是在对威海小石岛潮间带沉积物进行细菌资源调查时分离出的，公关中国。CH-27T菌株的细胞呈杆状，宽度为0.3-0.6µm，长度为2.0-11.0µm。菌株CH-27T在37°C时生长最佳，pH7.0和2.0%(w/v)NaCl。过氧化氢酶活性为弱阳性，氧化酶活性为阳性。基于16SrRNA基因序列的系统发育分析表明，菌株CH-27T与MarinihelvusfidelisKCTC92639T最相关（93.6％），其次是温州香菜码头MCCC1K00261T（92.0%）。基于菌株CH-27T和M.fidelisKCTC92639T之间的基因组比较，平均氨基酸同一性为63.6%,保守蛋白百分比为48.3%.菌株CH-27T（≥10％）的主要细胞脂肪酸是异C15：0，唯一的呼吸醌是醌8。极性脂质是磷脂酰甘油，磷脂酰乙醇胺，二磷脂酰甘油，和氨基磷脂.DNAG+C含量为62.7mol%。在综合分析其系统发育的基础上,生理,生物化学，和化学分类学特征，菌株CH-27T代表了一个新属中的新物种，这个名字叫Elongatibactersediminisgen。11月。,sp.11月。是提议的。菌株类型为CH-27T(=MCCC1H00480T=KCTC8011T)。
