Abstract：
BACKGROUND: Mild cognitive impairment (MCI) is a prodromal stage of dementia. Understanding the mechanistic changes from healthy aging to MCI is critical for comprehending disease progression and enabling preventative intervention.
METHODS: Patients with MCI and age-matched controls (CN) were administered cognitive tasks during functional near-infrared spectroscopy (fNIRS) recording, and changes in plasma levels of extracellular vesicles (EVs) were assessed using small-particle flow cytometry.
RESULTS: Neurovascular coupling (NVC) and functional connectivity (FC) were decreased in MCI compared to CN, prominently in the left-dorsolateral prefrontal cortex (LDLPFC). We observed an increased ratio of cerebrovascular endothelial EVs (CEEVs) to total endothelial EVs in patients with MCI compared to CN, correlating with structural MRI small vessel ischemic damage in MCI. LDLPFC NVC, CEEV ratio, and LDLPFC FC had the highest feature importance in the random Forest group classification.
CONCLUSIONS: NVC, CEEVs, and FC predict MCI diagnosis, indicating their potential as markers for MCI cerebrovascular pathology.
CONCLUSIONS: Neurovascular coupling (NVC) is impaired in mild cognitive impairment (MCI). Functional connectivity (FC) compensation mechanism is lost in MCI. Cerebrovascular endothelial extracellular vesicles (CEEVs) are increased in MCI. CEEV load strongly associates with cerebral small vessel ischemic lesions in MCI. NVC, CEEVs, and FC predict MCI diagnosis over demographic and comorbidity factors.
METHODS: Patients with MCI and age-matched controls (CN) were administered cognitive tasks during functional near-infrared spectroscopy (fNIRS) recording, and changes in plasma levels of extracellular vesicles (EVs) were assessed using small-particle flow cytometry.
RESULTS: Neurovascular coupling (NVC) and functional connectivity (FC) were decreased in MCI compared to CN, prominently in the left-dorsolateral prefrontal cortex (LDLPFC). We observed an increased ratio of cerebrovascular endothelial EVs (CEEVs) to total endothelial EVs in patients with MCI compared to CN, correlating with structural MRI small vessel ischemic damage in MCI. LDLPFC NVC, CEEV ratio, and LDLPFC FC had the highest feature importance in the random Forest group classification.
CONCLUSIONS: NVC, CEEVs, and FC predict MCI diagnosis, indicating their potential as markers for MCI cerebrovascular pathology.
CONCLUSIONS: Neurovascular coupling (NVC) is impaired in mild cognitive impairment (MCI). Functional connectivity (FC) compensation mechanism is lost in MCI. Cerebrovascular endothelial extracellular vesicles (CEEVs) are increased in MCI. CEEV load strongly associates with cerebral small vessel ischemic lesions in MCI. NVC, CEEVs, and FC predict MCI diagnosis over demographic and comorbidity factors.
摘要：
背景：轻度认知障碍（MCI）是痴呆的前驱阶段。了解从健康衰老到MCI的机制变化对于理解疾病进展和进行预防性干预至关重要。
方法：患有MCI和年龄匹配的对照组（CN）的患者在功能近红外光谱（fNIRS）记录期间接受认知任务，使用小粒子流式细胞术评估细胞外囊泡（EV）的血浆水平变化。
结果：与CN相比，MCI中的神经血管耦合（NVC）和功能连接（FC）降低，在左背外侧前额叶皮质（LDLPFC）中突出。我们观察到与CN相比，MCI患者的脑血管内皮EVs（CEEVs）与总内皮EVs的比率增加，与MCI中结构性MRI小血管缺血性损伤相关。LDLPFCNVC,CEEV比率，LDLPFCFC在随机森林组分类中具有最高的特征重要性。
结论：NVC，CEEV,并预测MCI诊断，表明它们作为MCI脑血管病理学标志物的潜力。
结论：神经血管偶联（NVC）在轻度认知障碍（MCI）中受损。功能连接(FC)补偿机制在MCI中丢失。MCI中脑血管内皮细胞外囊泡（CEEV）增加。CEEV负荷与MCI脑小血管缺血性病变密切相关。NVC,CEEV,并根据人口统计学和合并症因素预测MCI诊断。
方法：患有MCI和年龄匹配的对照组（CN）的患者在功能近红外光谱（fNIRS）记录期间接受认知任务，使用小粒子流式细胞术评估细胞外囊泡（EV）的血浆水平变化。
结果：与CN相比，MCI中的神经血管耦合（NVC）和功能连接（FC）降低，在左背外侧前额叶皮质（LDLPFC）中突出。我们观察到与CN相比，MCI患者的脑血管内皮EVs（CEEVs）与总内皮EVs的比率增加，与MCI中结构性MRI小血管缺血性损伤相关。LDLPFCNVC,CEEV比率，LDLPFCFC在随机森林组分类中具有最高的特征重要性。
结论：NVC，CEEV,并预测MCI诊断，表明它们作为MCI脑血管病理学标志物的潜力。
结论：神经血管偶联（NVC）在轻度认知障碍（MCI）中受损。功能连接(FC)补偿机制在MCI中丢失。MCI中脑血管内皮细胞外囊泡（CEEV）增加。CEEV负荷与MCI脑小血管缺血性病变密切相关。NVC,CEEV,并根据人口统计学和合并症因素预测MCI诊断。
