Abstract：
Despite advances in treating osteosarcoma, postoperative tumor recurrence, periprosthetic infection, and critical bone defects remain critical concerns. Herein, the growth of selenium nanoparticles (SeNPs) onto MgFe-LDH nanosheets (LDH) is reported to develop a multifunctional nanocomposite (LDH/Se) and further modification of the nanocomposite on a bioactive glass scaffold (BGS) to obtain a versatile platform (BGS@LDH/Se) for comprehensive postoperative osteosarcoma management. The uniform dispersion of negatively charged SeNPs on the LDH surface restrains toxicity-inducing aggregation and inactivation, thus enhancing superoxide dismutase (SOD) activation and superoxide anion radical (·O2 -)-H2O2 conversion. Meanwhile, Fe3+ within the LDH nanosheets can be reduced to Fe2+ by depleting glutathione (GSH) in the tumor microenvironments (TME), which can catalyze H2O2 into highly toxic reactive oxygen species. More importantly, incorporating SeNPs significantly promotes the anti-bacterial and osteogenic properties of BGS@LDH/Se. Thus, the developed BGS@LDH/Se platform can simultaneously inhibit tumor recurrence and periprosthetic infection as well as promote bone regeneration, thus holding great potential for postoperative \"one-stop-shop\" management of patients who need osteosarcoma resection and scaffold implantation.
摘要：
尽管在治疗骨肉瘤方面取得了进展，术后肿瘤复发,假体周围感染,和严重的骨缺损仍然是至关重要的问题。在这里，据报道，硒纳米颗粒（SeNPs）在MgFe-LDH纳米片（LDH）上的生长可开发出多功能纳米复合材料（LDH/Se），并在生物活性玻璃支架（BGS）上进一步修饰纳米复合材料，以获得多功能平台（BGS@LDH/Se），用于术后综合骨肉瘤管理。带负电荷的SeNPs在LDH表面上的均匀分散抑制了毒性诱导的聚集和失活，从而增强超氧化物歧化酶（SOD）的激活和超氧阴离子自由基（·O2-）-H2O2的转化。同时,通过消耗肿瘤微环境（TME）中的谷胱甘肽（GSH），可以将LDH纳米片中的Fe3还原为Fe2。可以将H2O2催化成高毒性的活性氧。更重要的是,掺入SeNPs显着促进BGS@LDH/Se的抗菌和成骨特性。因此，开发的BGS@LDH/Se平台可以同时抑制肿瘤复发和假体周围感染以及促进骨再生，因此，对需要骨肉瘤切除和支架植入的患者进行术后“一站式”管理具有巨大潜力。
