Abstract：
In this study, the bioactive components, enzyme inhibitory, antioxidant and anticancer potentials of edible (L. sativa) and a new species (L. anatolica) of Lactuca were evaluated and compared. The quantitative analyzes of the bioactive components of L. sativa (LS) and L. anatolica (LA) were analyzed quantitatively by GC-MS and Orbitrab HPLC-HRMS. Antioxidant, enzyme inhibitory and anticancer properties were analyzed by various assays. In general, LA exhibited more stronger antioxidant properties compared to LS. The extracts showed similar inhibitory effects on these enzymes. It was determined that LS was dominant in terms of linoleic acid (23.71%), while LA contained a high level of α-linolenic acid (31.70%). LA and LS inhibited the viability of A549 and MCF-7 cells in a dose-dependent manner. IC50 values for LA, LS and cisplatin were determined as 120.3, 197.5, 4.3 µg/mL in A549 cell line and 286.2, 472.8, 7.2 µg/mL in MCF-7 cell line, respectively. It was revealed that LA and LS treatment at 50 µg/mL concentrations in A549 cells completely suppressed the colony forming capacity, and treatment with IC50 doses inhibited cell migration, and triggered apoptosis by regulating caspase-3, cPARP, p53 and p21. The findings of this study suggested that these species have significant pharmacological potential.
摘要：
在这项研究中,生物活性成分，酶抑制性，可食用的抗氧化剂和抗癌潜力(L.sativa)和一个新物种(L.对Lactuca的anatolica）进行了评估和比较。通过GC-MS和OrbitrabHPLC-HRMS定量分析了紫花苜蓿（LS）和anatolica（LA）的生物活性成分。抗氧化剂,酶抑制和抗癌特性进行了分析，通过各种试验。总的来说,与LS相比,LA表现出更强的抗氧化性能。提取物对这些酶表现出相似的抑制作用。确定LS在亚油酸方面占主导地位（23.71％），而LA含有高水平的α-亚麻酸(31.70%)。LA和LS以剂量依赖性方式抑制A549和MCF-7细胞的活力。LA的IC50值，LS和顺铂在A549细胞系中测定为120.3、197.5、4.3µg/mL，在MCF-7细胞系中测定为286.2、472.8、7.2µg/mL，分别。揭示了在A549细胞中以50µg/mL浓度的LA和LS处理完全抑制了集落形成能力，用IC50剂量治疗抑制细胞迁移，并通过调节caspase-3，cPARP，p53和p21。这项研究的结果表明，这些物种具有显着的药理潜力。
