Abstract：
The juxtaposition of well-oxygenated intestinal colonic tissue with an anerobic luminal environment supports a fundamentally important relationship that is altered in the setting of intestinal injury, a process likely to be relevant to diseases such as inflammatory bowel disease. Herein, using two-color phosphorometry to non-invasively quantify both intestinal tissue and luminal oxygenation in real time, we show that intestinal injury induced by DSS colitis reduces intestinal tissue oxygenation in a spatially defined manner and increases the flux of oxygen from the tissue into the gut lumen. By characterizing the composition of the microbiome in both DSS colitis-affected gut and in a bioreactor containing a stable human fecal community exposed to microaerobic conditions, we provide evidence that the increased flux of oxygen into the gut lumen augments glycan degrading bacterial taxa rich in glycoside hydrolases which are known to inhabit gut mucosal surface. Continued disruption of the intestinal mucus barrier through such a mechanism may play a role in the perpetuation of the intestinal inflammatory process.
摘要：
氧合良好的肠结肠组织与厌氧性管腔环境的并置支持在肠损伤的设置中改变的根本重要关系，可能与炎症性肠病等疾病相关的过程。在这里，使用双色荧光法实时非侵入性地量化肠组织和腔内氧合，我们发现DSS结肠炎诱导的肠损伤以空间限定的方式降低了肠组织氧合,并增加了从组织进入肠腔的氧通量.通过表征DSS结肠炎影响的肠道和包含暴露于微氧条件的稳定人类粪便群落的生物反应器中的微生物组组成，我们提供的证据表明，进入肠腔的氧气通量增加增加了聚糖降解富含糖苷水解酶的细菌类群，已知这些糖苷水解酶栖息在肠粘膜表面。通过这种机制对肠粘液屏障的持续破坏可能在肠道炎症过程的延续中起作用。
