Abstract：
Aim: We investigate the genome-wide DNA methylation (DNAm) patterns of term low birth weight (TLBW) neonates. Methods: In the discovery phase, we assayed 32 samples (TLBW/control:16/16) using the EPIC 850k BeadChip Array. Targeted pyrosequencing of in 60 samples (TLBW/control:28/32) using targeted pyrosequencing during the replication phase. Results: The 850K array identified TLBW-associated 144 differentially methylated positions (DMPs) and 149 DMRs. Nearly 77% DMPs exhibited hypomethylation, located in the opensea and gene body regions. The most significantly enriched pathway in KEGG is sphingolipid metabolism (hsa00600), and the genes GALC and SGMS1 related to this pathway both show hypomethylation. Conclusion: Our analysis provides evidence of genome-wide DNAm alterations in TLBW. Further investigations are needed to elucidate the functional significance of these DNAm changes.
This study looked at the DNA of babies born after 37 weeks of pregnancy but weighing less than 2500 grams. We found that these babies had lower levels of DNA methylation, which might change how their bodies handle fats.
This study looked at the DNA of babies born after 37 weeks of pregnancy but weighing less than 2500 grams. We found that these babies had lower levels of DNA methylation, which might change how their bodies handle fats.
摘要：
目的：我们研究足月低出生体重（TLBW）新生儿的全基因组DNA甲基化（DNAm）模式。方法：在发现阶段，我们使用EPIC850kBeadChip阵列测定了32个样品(TLBW/对照:16/16)。在复制阶段期间使用靶向焦磷酸测序对60个样品(TLBW/对照:28/32)中的靶向焦磷酸测序。结果：850K阵列鉴定了TLBW相关的144个差异甲基化位置（DMPs）和149个DMRs。近77%的DMP表现出低甲基化，位于公海和基因体区域。KEGG中最显著的富集途径是鞘脂代谢(hsa00600)，与该途径相关的基因GALC和SGMS1均显示低甲基化。结论：我们的分析提供了TLBW全基因组DNAm改变的证据。需要进一步的研究来阐明这些DNAm变化的功能意义。
这项研究观察了怀孕37周后出生但体重不足2500克的婴儿的DNA。我们发现这些婴儿的DNA甲基化水平较低，这可能会改变他们的身体处理脂肪的方式。
这项研究观察了怀孕37周后出生但体重不足2500克的婴儿的DNA。我们发现这些婴儿的DNA甲基化水平较低，这可能会改变他们的身体处理脂肪的方式。
