PDF(Pubmed)
Abstract：
UNASSIGNED: Osteosarcoma (OS) is highly malignant and prone to local infiltration and distant metastasis. Due to the poor outcomes of OS patients, the study aimed to identify differentially expressed genes (DEGs) in OS and explore their role in the carcinogenesis and progression of OS.
UNASSIGNED: RNA sequencing was performed to identify DEGs in OS. The functions of the DEGs in OS were investigated using bioinformatics analysis, and DEG expression was verified using RT-qPCR and Western blotting. The role of SLC25A4 was evaluated using gene set enrichment analysis (GSEA) and then investigated using functional assays in OS cells.
UNASSIGNED: In all, 8353 DEGs were screened. GO and KEGG enrichment analyses indicated these DEGs showed strong enrichment in the calcium signaling pathway and pathways in cancer. Moreover, the Kaplan-Meier survival analysis showed ten hub genes were related to the outcomes of OS patients. Both SLC25A4 transcript and protein expression were significantly reduced in OS, and GSEA suggested that SLC25A4 was associated with cell cycle, apoptosis and inflammation. SLC25A4-overexpressing OS cells exhibited suppressed proliferation, migration, invasion and enhanced apoptosis.
UNASSIGNED: SLC25A4 was found to be significantly downregulated in OS patients, which was associated with poor prognosis. Modulation of SLC25A4 expression levels may be beneficial in OS treatment.
UNASSIGNED: RNA sequencing was performed to identify DEGs in OS. The functions of the DEGs in OS were investigated using bioinformatics analysis, and DEG expression was verified using RT-qPCR and Western blotting. The role of SLC25A4 was evaluated using gene set enrichment analysis (GSEA) and then investigated using functional assays in OS cells.
UNASSIGNED: In all, 8353 DEGs were screened. GO and KEGG enrichment analyses indicated these DEGs showed strong enrichment in the calcium signaling pathway and pathways in cancer. Moreover, the Kaplan-Meier survival analysis showed ten hub genes were related to the outcomes of OS patients. Both SLC25A4 transcript and protein expression were significantly reduced in OS, and GSEA suggested that SLC25A4 was associated with cell cycle, apoptosis and inflammation. SLC25A4-overexpressing OS cells exhibited suppressed proliferation, migration, invasion and enhanced apoptosis.
UNASSIGNED: SLC25A4 was found to be significantly downregulated in OS patients, which was associated with poor prognosis. Modulation of SLC25A4 expression levels may be beneficial in OS treatment.
摘要：
■骨肉瘤（OS）恶性程度高，易发生局部浸润和远处转移。由于OS患者的不良预后，本研究旨在鉴定OS中的差异表达基因（DEGs），并探讨其在OS发生发展中的作用。
■进行RNA测序以鉴定OS中的DEG。使用生物信息学分析研究了操作系统中DEG的功能，使用RT-qPCR和Western印迹验证DEG表达。使用基因集富集分析（GSEA）评估SLC25A4的作用，然后使用OS细胞中的功能测定进行研究。
■总之，筛选8353个DEGs。GO和KEGG富集分析表明这些DEGs在钙信号传导途径和癌症途径中显示出强烈的富集。此外，Kaplan-Meier生存分析显示,10个hub基因与OS患者的结局相关.SLC25A4转录物和蛋白质表达在OS中显著降低,GSEA提示SLC25A4与细胞周期相关，细胞凋亡和炎症。SLC25A4过表达的OS细胞表现出抑制的增殖，迁移,侵袭和增强细胞凋亡。
■在OS患者中发现SLC25A4显著下调，这与不良预后有关。调节SLC25A4表达水平在OS治疗中可能是有益的。
■进行RNA测序以鉴定OS中的DEG。使用生物信息学分析研究了操作系统中DEG的功能，使用RT-qPCR和Western印迹验证DEG表达。使用基因集富集分析（GSEA）评估SLC25A4的作用，然后使用OS细胞中的功能测定进行研究。
■总之，筛选8353个DEGs。GO和KEGG富集分析表明这些DEGs在钙信号传导途径和癌症途径中显示出强烈的富集。此外，Kaplan-Meier生存分析显示,10个hub基因与OS患者的结局相关.SLC25A4转录物和蛋白质表达在OS中显著降低,GSEA提示SLC25A4与细胞周期相关，细胞凋亡和炎症。SLC25A4过表达的OS细胞表现出抑制的增殖，迁移,侵袭和增强细胞凋亡。
■在OS患者中发现SLC25A4显著下调，这与不良预后有关。调节SLC25A4表达水平在OS治疗中可能是有益的。
