PDF(Pubmed)
Abstract：
Wolbachia continue to be reported in species previously thought to lack them, particularly Aedes aegypti mosquitoes. The presence of Wolbachia in this arbovirus vector is considered important because releases of mosquitoes with transinfected Wolbachia are being used around the world to suppress pathogen transmission and these efforts depend on a lack of Wolbachia in natural populations of this species. We previously assessed papers reporting Wolbachia in natural populations of Ae. aegypti and found little evidence that seemed convincing. However, since our review, more and more papers are emerging on Wolbachia detections in this species. Our purpose here is to evaluate these papers within the context of criteria we previously established but also new criteria that include the absence of releases of transinfections within the local areas being sampled which has contaminated natural populations in at least one case where novel detections have been reported. We also address the broader issue of Wolbachia detection in other insects where similar issues may arise which can affect overall estimates of this endosymbiont more generally. We note continuing shortcomings in papers purporting to find natural Wolbachia in Ae. aegypti which are applicable to other insects as well.
摘要：
沃尔巴克氏菌继续在以前被认为缺乏它们的物种中被报道，尤其是埃及伊蚊。这种虫媒病毒载体中Wolbachia的存在被认为是重要的，因为全世界都在使用具有被转染的Wolbachia的蚊子的释放来抑制病原体的传播，这些努力取决于该物种的自然种群中缺乏Wolbachia。我们以前评估了报告Wolbachia在Ae自然种群中的论文。埃及伊蚊，发现几乎没有证据，似乎令人信服。然而,自从我们审查以来，越来越多的论文出现在该物种的Wolbachia检测中。我们的目的是在我们先前建立的标准以及新标准的背景下评估这些论文，这些标准包括在至少一种报告有新检测的情况下，在被采样的局部区域内没有传播感染的释放，这些传播感染污染了自然人群。我们还解决了可能出现类似问题的其他昆虫中Wolbachia检测的更广泛问题，这些问题可能会更普遍地影响这种内共生体的总体估计。我们注意到旨在在Ae中找到天然Wolbachia的论文中存在持续的缺陷。埃及伊蚊也适用于其他昆虫。
