PDF(Pubmed)
Abstract：
In experimental allergic encephalomyelitis, the severity of the deficiency is associated with the loss of axons, and it is likely that cytotoxic T-cells 8 (CD8 T) play an important role. In relapsing-remitting multiple sclerosis, there is a correlation between the inflammatory activity in the lesion and the transection of axons. To understand the pathological mechanisms, it is important to evaluate the changes in serum concentrations of pro- and anti-inflammatory cytokines during the disease course. A total of 46 patients and 40 healthy individuals participated in an open-label, prospective, case-control study from 2012 to 2014. The serum concentrations of cytokines were measured using enzyme-linked immunosorbent assay (ELISA). An immune imbalance was observed during relapse and remission phases compared to the control group. During relapse, the levels of interferon-gamma (IFN-γ) were significantly higher compared to those in remission (p=0.017). During remission, there was an improvement in the deficiency (p<0.001), and the anti-inflammatory cytokines transforming growth factor-beta (TGF-β) and interleukin 4 (IL4) increased compared to those in relapse (p=0.006; p=0.009). A correlation was found between the serum concentrations of tumor necrosis factor-alpha (TNF-α) and Expanded Disability Status Scale (EDSS) during relapse (correlation coefficient: 0.301; significance (Sig.) (2-tailed 0.042). During the exacerbation, there was a moderate relationship between interleukin 17 (IL17) and 25-hydroxyvitamin D (25(OH)D) (P (p-value (probability value) = 0.02)). TNF-α, IFN-γ, IL17, and TGF-β serum levels are criteria for evaluating immune inflammatory activity during relapse and remission periods.
摘要：
在实验性过敏性脑脊髓炎中，缺乏的严重程度与轴突的丢失有关,细胞毒性T细胞8（CD8T）可能起重要作用。在复发缓解型多发性硬化症中，病变的炎症活动与轴突横断之间存在相关性。为了了解病理机制，评估疾病过程中血清促炎和抗炎细胞因子浓度的变化非常重要。共有46名患者和40名健康人参加了开放标签，prospective,2012-2014年病例对照研究。使用酶联免疫吸附测定(ELISA)测量细胞因子的血清浓度。与对照组相比，在复发和缓解期观察到免疫失衡。在复发期间，与缓解期相比，干扰素-γ（IFN-γ）水平显着升高（p=0.017）。在缓解期间,缺陷有所改善(p<0.001)，与复发患者相比，抗炎细胞因子转化生长因子-β（TGF-β）和白介素4（IL4）增加（p=0.006；p=0.009）。发现复发期间肿瘤坏死因子-α（TNF-α）的血清浓度与扩展的残疾状态量表（EDSS）之间存在相关性（相关系数：0.301；显著性（Sig。)(双尾0.042)。在恶化期间,白细胞介素17(IL17)和25-羟基维生素D(25(OH)D)之间存在中等关系(P(p值(概率值)=0.02)).TNF-α,IFN-γ，IL17和TGF-β血清水平是评估复发和缓解期免疫炎症活性的标准。
