Abstract：
OBJECTIVE: To compare the subgingival microbiota of patients receiving supportive periodontal care (SPC) with and without subgingival instrumentation, over 2 years.
METHODS: This study was a randomized clinical trial that included 62 participants (50.97 ± 9.26 years old; 40 females) who completed non-surgical periodontal therapy. Participants were randomly assigned to receive oral prophylaxis with oral hygiene instructions alone (test) or in combination with subgingival instrumentation (control) during SPC. Pooled subgingival biofilm samples were obtained from four sites per patient at SPC baseline and at 3, 6, 12, 18, and 24 months. Real-time polymerase chain reaction was used for absolute quantification of Eubacteria and the target bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Data were analysed using generalized estimating equations, taking into consideration the clustering of observations within individuals.
RESULTS: No significant differences were found between the experimental groups regarding the mean counts of Eubacteria and target bacteria, as well as the periodontal parameters at the sampled sites. Although significant variability in bacterial counts was present during SPC, all counts after 2 years were not statistically different from those at baseline. Bacterial counts were associated with the presence of plaque, bleeding on probing, mean probing depth ≥3 mm, and follow-up period.
CONCLUSIONS: SPC with or without subgingival instrumentation can result in comparable subgingival microbiological outcomes.
BACKGROUND: clinicaltrials.gov: NCT01598155 (https://clinicaltrials.gov/study/NCT01598155?intr=supragingival%20control&rank=4#study-record-dates).
METHODS: This study was a randomized clinical trial that included 62 participants (50.97 ± 9.26 years old; 40 females) who completed non-surgical periodontal therapy. Participants were randomly assigned to receive oral prophylaxis with oral hygiene instructions alone (test) or in combination with subgingival instrumentation (control) during SPC. Pooled subgingival biofilm samples were obtained from four sites per patient at SPC baseline and at 3, 6, 12, 18, and 24 months. Real-time polymerase chain reaction was used for absolute quantification of Eubacteria and the target bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Data were analysed using generalized estimating equations, taking into consideration the clustering of observations within individuals.
RESULTS: No significant differences were found between the experimental groups regarding the mean counts of Eubacteria and target bacteria, as well as the periodontal parameters at the sampled sites. Although significant variability in bacterial counts was present during SPC, all counts after 2 years were not statistically different from those at baseline. Bacterial counts were associated with the presence of plaque, bleeding on probing, mean probing depth ≥3 mm, and follow-up period.
CONCLUSIONS: SPC with or without subgingival instrumentation can result in comparable subgingival microbiological outcomes.
BACKGROUND: clinicaltrials.gov: NCT01598155 (https://clinicaltrials.gov/study/NCT01598155?intr=supragingival%20control&rank=4#study-record-dates).
摘要：
目的：比较使用和不使用牙龈下器械的牙周支持性护理（SPC）患者的牙龈下微生物群，超过2年。
方法：这项研究是一项随机临床试验，包括62名完成非手术牙周治疗的参与者（50.97±9.26岁；40名女性）。在SPC期间，参与者被随机分配接受口腔预防，仅使用口腔卫生说明（测试）或与龈下器械（对照）结合使用。在SPC基线和3、6、12、18和24个月时,从每个患者的四个部位获得汇集的龈下生物膜样品。实时聚合酶链反应用于对真细菌和目标细菌牙龈卟啉单胞菌进行绝对定量，连翘坦菌,和Denticola密螺旋体.使用广义估计方程分析数据，考虑到个体内部观察的聚类。
结果：实验组之间在真细菌和目标细菌的平均计数方面没有发现显着差异，以及采样部位的牙周参数。尽管在SPC期间存在细菌计数的显着差异，2年后的所有计数与基线无统计学差异.细菌计数与斑块的存在有关，探查时出血,平均探测深度≥3mm,和随访期。
结论：SPC有或没有龈下器械可以导致相当的龈下微生物学结果。
背景：clinicaltrials.gov:NCT01598155(https://clinicaltrials.gov/study/NCT01598155?intr=牙龈上%20control&rank=4#研究记录日期)。
方法：这项研究是一项随机临床试验，包括62名完成非手术牙周治疗的参与者（50.97±9.26岁；40名女性）。在SPC期间，参与者被随机分配接受口腔预防，仅使用口腔卫生说明（测试）或与龈下器械（对照）结合使用。在SPC基线和3、6、12、18和24个月时,从每个患者的四个部位获得汇集的龈下生物膜样品。实时聚合酶链反应用于对真细菌和目标细菌牙龈卟啉单胞菌进行绝对定量，连翘坦菌,和Denticola密螺旋体.使用广义估计方程分析数据，考虑到个体内部观察的聚类。
结果：实验组之间在真细菌和目标细菌的平均计数方面没有发现显着差异，以及采样部位的牙周参数。尽管在SPC期间存在细菌计数的显着差异，2年后的所有计数与基线无统计学差异.细菌计数与斑块的存在有关，探查时出血,平均探测深度≥3mm,和随访期。
结论：SPC有或没有龈下器械可以导致相当的龈下微生物学结果。
背景：clinicaltrials.gov:NCT01598155(https://clinicaltrials.gov/study/NCT01598155?intr=牙龈上%20control&rank=4#研究记录日期)。
