Abstract：
Mefenamic acid, renowned for its analgesic properties, stands as a reliable choice for alleviating mild to moderate pain. However, its versatility extends beyond pain relief, with ongoing research unveiling its promising therapeutic potential across diverse domains. A straightforward, environmentally friendly, and sensitive spectrofluorometric technique has been developed for the precise quantification of the analgesic medication, mefenamic acid. This method relies on the immediate reduction of fluorescence emitted by a probe upon interaction with varying concentrations of the drug. The fluorescent probe utilized, N-phenyl-1-naphthylamine (NPNA), was synthesized in a single step, and the fluorescence intensities were measured at 480 nm using synchronous fluorescence spectroscopy with a wavelength difference of 200 nm. Temperature variations and lifetime studies indicated that the quenching process was static. The calibration curve exhibited linearity within the concentration range of 0.50-9.00 μg/mL, with a detection limit of 60.00 ng/mL. Various experimental parameters affecting the quenching process were meticulously examined and optimized. The proposed technique was successfully applied to determine mefenamic acid in pharmaceutical formulations, plasma, and urine, yielding excellent recoveries ranging from 98% to 100.5%. The greenness of the developed method was evaluated using three metrics: the Analytical Eco-scale, AGREE, and the Green Analytical Procedure Index.
摘要：
甲芬那酸,以其镇痛特性而闻名，作为缓解轻度至中度疼痛的可靠选择。然而,它的多功能性超越了疼痛缓解，正在进行的研究揭示了其在不同领域的有前途的治疗潜力。一个直截了当的，环保,和灵敏的荧光光谱技术已经开发用于精确定量镇痛药物，甲芬那酸.该方法依赖于探针在与不同浓度的药物相互作用时发射的荧光的立即减少。利用的荧光探针，N-苯基-1-萘胺(NPNA),一步合成，使用波长差为200nm的同步荧光光谱法在480nm处测量荧光强度。温度变化和寿命研究表明，淬火过程是静态的。校准曲线在0.50-9.00μg/mL浓度范围内呈线性关系,检测限为60.00ng/mL。精心检查和优化了影响淬火过程的各种实验参数。所提出的技术已成功应用于测定药物制剂中的甲芬那酸，等离子体,和尿液，回收率从98%到100.5%不等。所开发方法的绿色性使用三个指标进行评估：分析生态尺度，同意,和绿色分析程序指数。
