METHODS: This study focuses on optimising the STP extraction process to ensure consistency in the quality of the product obtained through ancient and modern processes using the standard relation and fuzzy analytic hierarchical process (FAHP) and criteria importance through intercriteria correlation (CRITIC) method integrated weights combined with the Box-Behnken response surface test. Using the contents of rosmarinic acid, isoimperatorin, puerarin, as well as the extract yield and fingerprint similarity as evaluation indexes of STP, the Box-Behnken response surface method was employed to examine the varying extraction parameters, including water addition ratio, extraction duration, and number of extractions. The weighted coefficients for each parameter were calculated by combining the benchmark correlation and FAHP-CRITIC method, deriving a comprehensive score.
RESULTS: The optimal extraction process for STP consisted of a two extractions, each using at a tenfold quantity of water, performed for one hour. Process verification across three separate batches yielded a comprehensive score of 94.7, with a relative standard deviation of 0.76%.
CONCLUSIONS: The application of the Box-Behnken response surface method combined with standard relation and FAHP-CRITIC approach proved to be stable and feasible for optimising the extraction process of STP.
方法:本研究的重点是优化STP提取过程,以确保通过使用标准关系和模糊层次分析法(FAHP)和重要性标准通过标准间相关性(CRITIC)方法集成权重结合Box-Behnken响应面测试,通过古代和现代工艺获得的产品质量的一致性。使用迷迭香酸的含量,异欧前胡素,葛根素,以提取物得率和指纹图谱相似度作为STP的评价指标,采用Box-Behnken响应面法检查了不同的提取参数,包括水添加比,提取持续时间,和提取次数。通过结合基准相关性和FAHP-CRITIC方法计算每个参数的加权系数,得出一个综合评分。
结果:STP的最佳提取工艺包括两次提取,每次使用十倍量的水,表演了一个小时。三个单独批次的过程验证得出的综合评分为94.7,相对标准偏差为0.76%。
结论:应用Box-Behnken响应面法结合标准关系法和FAHP-CRITIC方法对优化STP的提取工艺是稳定可行的。