PDF(Pubmed)
Abstract：
OBJECTIVE: The fresh-market tomato (Solanum lycopersicum) is bred for direct human consumption. It is selected for specific traits to meet market demands and production systems, and unique genetic variations underlying fresh-market tomato yields have been recently identified. However, DNA sequence variant-trait associations are not yet fully examined even for major traits. To provide a rich genome sequence resource for various genetics and breeding goals for fresh-market tomato traits, we report whole genome sequence data of a pool of contemporary U.S. fresh-market tomatoes.
METHODS: Eighty-one tomatoes were nominated by academic tomato breeding programs in the U.S. Of the 81 tomatoes, 68 were contemporary fresh-market tomatoes, whereas the remaining 13 were relevant fresh-market tomato breeding and germplasm accessions. Whole genome sequencing (WGS) of the 81 tomatoes was conducted using the Illumina next-generation sequencing technology. The polymerase chain reaction (PCR)-free, paired-end sequencing libraries were sequenced on an average depth per sequenced base of 24 × for each tomato. This data note enhances visibility and potential for use of the more diverse, freely accessible whole genome sequence data of contemporary fresh-market tomatoes.
METHODS: Eighty-one tomatoes were nominated by academic tomato breeding programs in the U.S. Of the 81 tomatoes, 68 were contemporary fresh-market tomatoes, whereas the remaining 13 were relevant fresh-market tomato breeding and germplasm accessions. Whole genome sequencing (WGS) of the 81 tomatoes was conducted using the Illumina next-generation sequencing technology. The polymerase chain reaction (PCR)-free, paired-end sequencing libraries were sequenced on an average depth per sequenced base of 24 × for each tomato. This data note enhances visibility and potential for use of the more diverse, freely accessible whole genome sequence data of contemporary fresh-market tomatoes.
摘要：
目的：新鲜市场番茄（Solanumlycopersicum）是为人类直接食用而培育的。它是针对特定特性选择的，以满足市场需求和生产系统，最近发现了新鲜市场番茄产量的独特遗传变异。然而,即使对于主要性状，DNA序列变异-性状关联也尚未完全检查。为新鲜番茄性状的各种遗传育种目标提供丰富的基因组序列资源,我们报告了当代美国新鲜番茄的全基因组序列数据.
方法：美国学术番茄育种计划提名了81个番茄，68个是当代新鲜市场的西红柿，而其余13个是相关的新鲜番茄育种和种质材料。使用Illumina下一代测序技术对81个西红柿进行了全基因组测序（WGS）。无聚合酶链反应（PCR），对每个番茄的配对末端测序文库进行测序,每个测序碱基的平均深度为24倍.本数据说明提高了知名度和使用更多样化的潜力，免费获取当代新鲜市场西红柿的全基因组序列数据。
方法：美国学术番茄育种计划提名了81个番茄，68个是当代新鲜市场的西红柿，而其余13个是相关的新鲜番茄育种和种质材料。使用Illumina下一代测序技术对81个西红柿进行了全基因组测序（WGS）。无聚合酶链反应（PCR），对每个番茄的配对末端测序文库进行测序,每个测序碱基的平均深度为24倍.本数据说明提高了知名度和使用更多样化的潜力，免费获取当代新鲜市场西红柿的全基因组序列数据。
