PDF(Pubmed)
Abstract：
In order to resolve the key genes for weed control by Trichoderma polysporum at the genomic level, we extracted the genomic DNA and sequenced the whole genome of T. polysporum strain HZ-31 on the Illumina Hiseq platform. The raw data was cleaned up using Trimmomatic and checked for quality using FastQC. The sequencing data was assembled using SPAdes, and GeneMark was used to perform gene prediction on the assembly results. The results showed that the genome size of T. polysporum HZ-31 was 39,325,746 bp, with 48% GC content, and the number of genes encoded was 11,998. A total of 148 tRNAs and 45 rRNAs were predicted. A total of 782 genes were annotated in the Carbohydrase Database, 757 genes were annotated to the Pathogen-Host Interaction Database, and 67 gene clusters were identified. In addition, 1023 genes were predicted to be signal peptide proteins. The annotation and functional analysis of the whole genome sequence of T. polymorpha HZ-31 provide a basis for the in-depth study of the molecular mechanism of its herbicidal action and more effective utilization for weed control.
摘要：
为了在基因组水平上解析多孢木霉控制杂草的关键基因,我们在IlluminaHiseq平台上提取了基因组DNA并测序了多孢菌菌株HZ-31的全基因组。使用Trimmoatic清理原始数据，并使用FastQC检查质量。使用SPAdes组装测序数据，并利用GeneMark对装配结果进行基因预测。结果表明，多孢子菌HZ-31的基因组大小为39,325,746bp，具有48%的GC含量,编码的基因数量为11,998个。预测了总共148个tRNA和45个rRNA。在碳水化合物酶数据库中总共注释了782个基因，757个基因被注释到病原体-宿主相互作用数据库中，并鉴定出67个基因簇。此外,预测1023个基因是信号肽蛋白。多形虫HZ-31全基因组序列的注释和功能分析为深入研究其除草作用的分子机制和更有效的利用杂草控制提供了基础。
